National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Antagonistic potentiality of four isolates of Trichoderma species viz. Trichoderma viride isolate A, Trichoderma viride isolate M, Trichoderma harzianum isolate C and Trichoderma harzianum isolate L against Beauveria bassiana causing white muscardine disease of Silk worm, Bombyx mori L, was studied invitro by studying the mycelial interaction between them as well as by the effect of culture filtrate of biocontrol agents on sporulation and spore germination of pathogen. Trichoderma viride isolate A and Trichoderma viride isolate M produced the maximum inhibition zone of 68.00 and 44.25 mm area respectively, followed by Trichoderma harzianum isolate L with inhibition zone of 39.60 mm and Trichoderma harzianum isolate C with 33.50 mm. In dual culture all the isolates of Trichoderma spp. over grew the pathogen and covered the entire medium surface. Its mycelia coiled around the hyphae of test pathogen and finally disintegrated it. The inhibition in mycelial growth by the cultural filtrate of Trichoderma harzianum isolate L was 55.35 %. The cultural filtrate of Trichoderma viride isolate A has inhibited the spore production and germination by 63.65 and 83.70 % respectively. The mechanism of antagonism was found to be hyper parasitism and antibiosis. The studies conducted revealed that all the tested biocontrol agents possessed antagonistic properties against Beauveria bassiana in vitro and could be used in controlling the white muscardine disease of silkworm.
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