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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(4): 945-951
DOI: https://doi.org/10.20546/ijcmas.2020.904.113


In vitro Evaluation of Trichoderma Species for the Biological Control of Beauveria bassiana (Balls) Vuill Causing White Muscardine Disease of Silk Worm, Bombyx mori L. under Temperate Conditions
Haroon Rashid Ahmad* and Gulzar Ahmad
Central Sericultural Research and Training Institute Pampore, Post bag 88 GPO Srinagar 190001(Jammu & Kashmir UT), India
*Corresponding author
Abstract:

Antagonistic potentiality of four isolates of Trichoderma species viz. Trichoderma viride isolate A, Trichoderma viride isolate M, Trichoderma harzianum isolate C and Trichoderma harzianum isolate L against Beauveria bassiana causing white muscardine disease of Silk worm, Bombyx mori L, was studied invitro by studying the mycelial interaction between them as well as by the effect of culture filtrate of biocontrol agents on sporulation and spore germination of pathogen. Trichoderma viride isolate A and Trichoderma viride isolate M produced the maximum inhibition zone of 68.00 and 44.25 mm area respectively, followed by Trichoderma harzianum isolate L with inhibition zone of 39.60 mm and Trichoderma harzianum isolate C with 33.50 mm. In dual culture all the isolates of Trichoderma spp. over grew the pathogen and covered the entire medium surface. Its mycelia coiled around the hyphae of test pathogen and finally disintegrated it. The inhibition in mycelial growth by the cultural filtrate of Trichoderma harzianum isolate L was 55.35 %. The cultural filtrate of Trichoderma viride isolate A has inhibited the spore production and germination by 63.65 and 83.70 % respectively. The mechanism of antagonism was found to be hyper parasitism and antibiosis. The studies conducted revealed that all the tested biocontrol agents possessed antagonistic properties against Beauveria bassiana in vitro and could be used in controlling the white muscardine disease of silkworm.


Keywords: Bombyx mori L, White muscardine, Beauveria bassiana, Antagonism, Trichoderma spp.
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How to cite this article:

Haroon Rashid Ahmad and Gulzar Ahmad. 2020. In vitro Evaluation of Trichoderma Species for the Biological Control of Beauveria bassiana (Balls) Vuill Causing White Muscardine Disease of Silk Worm, Bombyx mori L. under Temperate Conditions.Int.J.Curr.Microbiol.App.Sci. 9(4): 945-951. doi: https://doi.org/10.20546/ijcmas.2020.904.113