Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(4): 686-705
DOI: https://doi.org/10.20546/ijcmas.2020.904.083


Immunological and Cytopathological Assessment of Trichomonas vaginalis Infection in Asymptomatic and Symptomatic Females at Menoufia Governorate, Egypt
Asmaa R. Hegazy1, Wafaa M. El Kersh1, Ismail M. Moharm1, Amany I. Ammar1*, Aiat S. Hemida2 and Amany F. Atia1
1Medical Parasitology, Faculty of Medicine, Menoufia University, Egypt
2Pathology department, Faculty of Medicine, MenoufiaUniversity, Egypt
*Corresponding author
Abstract:

Trichomonas vaginalis (T. vaginalis) is a common health problem all over the world. The present study aimed to determine the prevalence rate and risk factors of T. vaginalis in females in Menoufia Governorate, Egypt and assess some immunological parameters includingIL-2, IL-17, IL22 and INFᵧ and their relation to cytopathological changes in asymptomatic and symptomatic T. vaginalis infected females. Two hundred females aging between 18 and 50 years' old were classified into two groups: Group I: Asymptomatic females subdivided into subgroup I a (non-infected) from which control subgroup were selected and I b (infected).Group II: Symptomatic females was subdivided into subgroup II a (non-infected) and II b (infected).The groups were screened for T.vaginalis by direct wet mount, Giemsa, Acridine orange (AO) and Papanicolaou (Pap) stained vaginal smears and culture technique. Cytopathological examination was done by using pap smears. Immunological detection of vaginal IL-2, IL-17, IL-22 and IFNγ were assessed using enzyme linked immunosorbent assay (ELISA). The results revealed that the prevalence rate of T. vaginalis was 22.5% (45/200) among the studied participants as proved by culture method, 25% (25/100) in asymptomatic females and 20% (20/100) in symptomatic females. There were significant elevated levels of IL-2, IL-17, IL-22 and IFN-γ in asymptomatic infected subgroup(Ib) than symptomatic infected subgroup (IIb) and control subgroup and decrease in cytopathological changes including halo cells, reactive nuclear changes and ghost cell. Elevated cytokines play a protective effect in asymptomatic T.vaginalis infected females proved by histopathological inflammatory reaction and decrease symptoms in these females.


Keywords: Trichomonas vaginalis, Menoufia, Cytokines, IL-22, IL-17, IL-2, IFN-γ, Cytopathological changes
Download this article as Download

How to cite this article:

Asmaa R. Hegazy, Wafaa M. El Kersh, Ismail M. Moharm, Amany I. Ammar, Aiat S. Hemida and Amany F. Atia. 2020. Immunological and Cytopathological Assessment of Trichomonas vaginalis Infection in Asymptomatic and Symptomatic Females at Menoufia Governorate, Egypt.Int.J.Curr.Microbiol.App.Sci. 9(4): 686-705. doi: https://doi.org/10.20546/ijcmas.2020.904.083