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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(3): 2699-2709
DOI: https://doi.org/10.20546/ijcmas.2020.903.308


Antibiogram and Diversity of Extended-Spectrum Beta-Lactamase Genes in Scavenging Local Chicken in Morogoro Municipality, Tanzania
Emmanuel Odartei Armah1* and Huruma Nelkiwe Tuntufye2
1Biomedical and Public Health Research Unit, Water Research Institute, CSIR, Ghana
2Department of Microbiology, Parasitology and Immunology, College of Veterinary and Medical Sciences, Sokoine University of Agriculture,Morogoro, Tanzania
*Corresponding author
Abstract:

The poultry industry, especially chicken production has in recent times faced a major set-back due to devastating effects of APEC Extended Spectrum Beta-Lactases (ESBL) producing organisms. This research aimed at investigating antimicrobial susceptibility profile and diversity of ESBL producing avian pathogenic Escherichia coli(APEC) among fecal cloacal swaps of scavenging local chickens based on the various housing systems. The APEC isolates were determined by virulence factor profiling and by Kirby-Baeur disc diffusion, 42% of the APEC isolates were found to be ESBL producers. Of the ESBL isolates, 87.5% were resistant to nalidixc acid, 37.5% were resistant to cefotaxime, Trimethroprim-Sulfamethoxazole, augmentnin and cephalothin, 25% were resistant to Ceftriaxone whiles no isolate was resistant to Imipenem, gentamycin and ciprofloxacin. On screening, a total of 32 beta-lactamase genes were found amongst these isolates, all of these isolates harbored the blaTEM gene.Semi-intensively kept chickens harbored more ESBL genes and in more diverse forms than the extensively kept ones.


Keywords: ESBL, APEC, CTX-M, TEM, Morogoro
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How to cite this article:

Emmanuel Odartei Armah and Huruma Nelkiwe Tuntufye. 2020. Antibiogram and Diversity of Extended-Spectrum Beta-Lactamase Genes in Scavenging Local Chicken in Morogoro Municipality, Tanzania.Int.J.Curr.Microbiol.App.Sci. 9(3): 2699-2709. doi: https://doi.org/10.20546/ijcmas.2020.903.308