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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(3): 2643-2655
DOI: https://doi.org/10.20546/ijcmas.2020.903.302


Characterization of Diazotrophs Isolated from Sugarcane Rhizosphere in Different Regions of Bangladesh
G. M. A. Hossain1*, A. R. M. Solaiman2, A. J. M. S. Karim2, G. K. M. M. Rahman2, M. A. Baset3, M. M. Rahman4 and S. Dev4
1Bangladesh Sugarcrop Research Institute, Ishurdi-6620, Pabna, Bangladesh
2Department of Soil Science
3Department of Crop Botany
4Department of Biotechnology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Salna, Gazipur-1706, Bangladesh
*Corresponding author
Abstract:

The experiment was conducted at the Soil Microbiology Laboratory of the Department of Soil Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur during the period from January, 2011 to June, 2011 with a view to isolate and characterized promising diazotrophs from sugarcane rhizosphere. Twenty four bacterial strains were isolated from sugarcane rhizosphere soils. Among the 24 isolated strains thirteen of them were found as Gram negative and eleven were Gram positive. Seventeen of the strains were positive for cellulose activity. Fifteen of the strains were found as positive for phosphate solubilisation activity. All of these bacterial strains were evaluated for nitrogen fixation activity, among them 21 isolates were found as ARA positive. The highest ARA was found in strain Acinetobacter calcoaciticus (BUSo 9) (3.74 x 10-4 μmol C2H4 cfu-1hr-1) which was statistically similar to Bacillus cereus (BUSo 13) (1.54 x 10-4 μmol C2H4 cfu-1hr-1). All of the strains were found as IAA producing. Two promising strains were selected for 16SrDNA sequencing. From 16S rDNA sequence analysis of the strains it was observed that ninety nine per cent sequence identity was observed between the 16S rDNA sequence of BUSo 9 and Acinetobacter calcoaciticus and also ninety nine per cent sequence identity was observed between the 16S rDNA sequence of BUSo 13 and Bacillus cereus.


Keywords: Characterization, Diazotrophs, Sugarcane, Rhizosphere, Bangladesh
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How to cite this article:

Hossain, G. M. A., A. R. M. Solaiman, A. J. M. S. Karim, G. K. M. M. Rahman, M. A. Baset, M. M. Rahman and Dev, S. 2020. Characterization of Diazotrophs Isolated from Sugarcane Rhizosphere in Different Regions of Bangladesh.Int.J.Curr.Microbiol.App.Sci. 9(3): 2643-2655. doi: https://doi.org/10.20546/ijcmas.2020.903.302