National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The experiment was conducted at the Soil Microbiology Laboratory of the Department of Soil Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur during the period from January, 2011 to June, 2011 with a view to isolate and characterized promising diazotrophs from sugarcane rhizosphere. Twenty four bacterial strains were isolated from sugarcane rhizosphere soils. Among the 24 isolated strains thirteen of them were found as Gram negative and eleven were Gram positive. Seventeen of the strains were positive for cellulose activity. Fifteen of the strains were found as positive for phosphate solubilisation activity. All of these bacterial strains were evaluated for nitrogen fixation activity, among them 21 isolates were found as ARA positive. The highest ARA was found in strain Acinetobacter calcoaciticus (BUSo 9) (3.74 x 10-4 μmol C2H4 cfu-1hr-1) which was statistically similar to Bacillus cereus (BUSo 13) (1.54 x 10-4 μmol C2H4 cfu-1hr-1). All of the strains were found as IAA producing. Two promising strains were selected for 16SrDNA sequencing. From 16S rDNA sequence analysis of the strains it was observed that ninety nine per cent sequence identity was observed between the 16S rDNA sequence of BUSo 9 and Acinetobacter calcoaciticus and also ninety nine per cent sequence identity was observed between the 16S rDNA sequence of BUSo 13 and Bacillus cereus.
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