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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(3): 2484-2489
DOI: https://doi.org/10.20546/ijcmas.2020.903.284


Status of Total Lymphocytes and T-Lymphocytes Population in Healthy Broiler Chicks
Kalpana Suman1* and Arun Prasad2
1Department of Biotechnology, Sai Mahavidyalaya (Bhilai), Pt.Ravishankar Shukla University, Raipur (Chhattisgarh) India
2Department of Veterinary Microbiology, Ranchi Veterinary College, Kanke, Ranchi (Jharkhand), India
*Corresponding author
Abstract:

The present study was carried out on 3-4 weeks old pathogen free broiler chicks (6 in numbers), maintained on standard feed from Ranchi Veterinary College (BAU) poultry farm . During the total lymphocyte count, lymphocytes appeared very distinctly from monocytes obtained from white buffy coat of mononuclear cells after centrifugation with Ficoll-paque by trypan blue dye exclusion technique with the help of haemocytometer. T-lymphocytes/T-cells showed characteristic greenish yellow fluorescence when stained with FITC (1:10 dilution) tagged antiserum raised against thymocytes. The mean of total lymphocytes & T-lymphocytes of 6 chicks taken as the blood values for time interval of one week on all days of examination were found to be highly significant (p<0.01). T-lymphocytes were found to be about 70% of total lymphocytes in healthy chicks in all days of examination persistently; although their number (total lymphocytes and total T-lymphocytes) increases with age.


Keywords: Total lymphocyte, T-lymphocyte, Immune system, Broiler chicks.
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How to cite this article:

Kalpana Suman and Arun Prasad. 2020. Status of Total Lymphocytes and T-Lymphocytes Population in Healthy Broiler Chicks.Int.J.Curr.Microbiol.App.Sci. 9(3): 2484-2489. doi: https://doi.org/10.20546/ijcmas.2020.903.284