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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(3): 2432-2439
DOI: https://doi.org/10.20546/ijcmas.2020.903.278


Novel T Cell Epitope Designing from PPRV HN Protein for Peptide based Subunit Vaccine: An Immune Informatics Approach
Aditya Agrawal1*, Rohini Gupta2, Anil Gattani1, Shailesh Kumar Patel3, Mahvash Hira Khan1 and Praveen Singh1
1Division of Animal Biochemistry, IVRI, Izatnagar, Bareilly, U.P. India
2Division of Medicine, IIVER, Rohtak, Haryana, India
3Division of Pathology, IVRI, Izatnagar, Bareilly, U.P. INDIA, India
*Corresponding author
Abstract:

Peste-des-petits Ruminants (PPR) is a disease of small ruminants especially goat and its control and eradication till 2030 wants an extensive research to develop a potent vaccine. The role of surface HN protein in the attachment of the virus to cellular receptors makes it an appropriate target to develop a theranostics against the virus. In this study, cytotoxic T cells epitopes that will bind to MHC class I alleles were predicted out using bioinformatic tools. Ten immunogenic peptides were predicted using IEDB web server based on their binding with cow (BoLA) alleles. Among these predicted peptides, five immunogenic epitopes i.e. 429SVFGPLIPHL438, 86HQTKDVLTPL95, 261RDLGLGPPVF270, 432GPLIPHLSGM441 and 555VRLNFKGNPL564 were selected on the basis of their high percentile score. Predicted three dimensional (3D) models of the PPRV HN protein and SLAM receptor were built and used to dock the immunogenic epitopes. It was used to predict the docked site in the structure. Furthermore, the involvement of these predicted epitopes in experiments may lead to creation of novel potent vaccine and diagnostic tools against the PPR.


Keywords: PPR virus, HN protein, T cell epitope, MHC
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How to cite this article:

Aditya Agrawal, Rohini Gupta, Anil Gattani, Shailesh Kumar Patel, Mahvash Hira Khan and Praveen Singh. 2020. Novel T Cell Epitope Designing from PPRV HN Protein for Peptide based Subunit Vaccine: An Immune Informatics Approach.Int.J.Curr.Microbiol.App.Sci. 9(3): 2432-2439. doi: https://doi.org/10.20546/ijcmas.2020.903.278