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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(3): 2117-2130
DOI: https://doi.org/10.20546/ijcmas.2020.903.242


Production, Characterization and Cytotoxic Evaluation of Pyocyanin Pigment Extracted from Pseudomonas aeruginosa Isolated from Industrial Soil Resources
Indumathi Mullaiselvan1, Vijayarani Kanagaraj2*, Baskaran Dharmar3, Murugan Balaraman4 and Sundaram Meignanalakshmi5
1Doctorate Programme of  Food Technology, Department of Food Processing Technology, 4Faculty of  Food safety and Quality assurance, College of Food and Dairy Technology, Tamil Nadu Veterinary and Animal Sciences University, Chennai, India 600 051.
2Faculty of Bioinformatics Centre and ARIS cell, 5Faculty of Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, India 600 007
3Faculty of Dairy Science, Department of Live stocks Products Technology Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, India 600 007
*Corresponding author
Abstract:

Thirty-five isolates belonging to the various genus of bacteria family were isolated from 25 different soil samples from various industrial areas from different districts of Tamil Nadu. One out of them were identified as P.aeruginosa (P1) and screened for pyocyanin production using submerged fermentation. The Pyocyanin production reached µg/ml on modified pseudomonas broth. The identification of strain was confirmed by 16s rRNA, the similarity with other strains available in the database was 98% (FDAARGOS_767). P1 was accessed at gene bank with accession number CP041008.1. Identification of the PhzM gene encoding PhzM enzyme was carried out using gene-specific primer followed by PCR. 190bp size gene was amplified using PCR. PhzM gene was confirmed and similarity with (FDAARGOS_767) strain was 100% in the database. Pyocyanin was extracted by standard chloroform extraction method, purified by HPLC (C17 column) and characterized by UV-Vis absorption spectroscopy, Particle size, zeta potential, FT-IR and LC-MS. The SEM analysis of pyocyanin pigment showed the particle size of microencapsulated pyocyanin. The cytotoxic effect of pyocyanin was investigated using VERO cells and it showed a concentration-response relationship between the concentration and cell viability the cytotoxicity increased gradually with the increase of its concentration.


Keywords: Cytotoxic evaluation, Pyocyanin, Pseudomonas aeruginosa Industrial Soil Resources
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How to cite this article:

Indumathi Mullaiselvan, Vijayarani Kanagaraj, Baskaran Dharmar, Murugan Balaraman and Sundaram Meignanalakshmi. 2020. Production, Characterization and Cytotoxic Evaluation of Pyocyanin Pigment Extracted from Pseudomonas aeruginosa Isolated from Industrial Soil Resources.Int.J.Curr.Microbiol.App.Sci. 9(3): 2117-2130. doi: https://doi.org/10.20546/ijcmas.2020.903.242