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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(3): 2026-2056
DOI: https://doi.org/10.20546/ijcmas.2020.903.235


A Decade of Tomato Transcriptomics: Status and Perspectives
Kinjal Kulshresthaand Akarsh Parihar
Centre of Excellence in Biotechnology, Department of Agricultural Biotechnology, Anand Agricultural University, Anand-388110, Gujarat, India
*Corresponding author
Abstract:

Tomato is widely consumed vegetable in the world. It is used as raw and processed form. Tomato flowers are self as well as controlled pollinated. The crop has more than thirteen species including cultivated and wild types with huge diversity and is susceptible to diverse stress conditions. Recent advances in the biotic and abiotic stress in tomato transcriptomics have been reviewed here. Transcriptome is a particular set of RNA expressed during a stress, developmental stages or a condition in a group of cells. Transcriptomics is a promising technology for understanding molecular interactions during disease, interpretation of functional elements and molecular constituents of cell. Transcriptomics allows indexing all transcripted species such as mRNA & small RNA; to determine gene structure: start site, 5’and 3’ends, transcriptional modification, splicing patterns; and to quantify differential gene expression in stress condition. There are many techniques of transcriptomics such as RNA sequencing, Microarray, EST and SAGE. Transcriptomics by RNASeq is frequently used system as being cost effective. Sample preparation is a critical step for sequencing procedure. High quality RNA is essential. As transcriptomics yield huge amount of datasets, bioinformatics tools are required for proper data analysis. Gene ontology is obtained as molecular function, cellular components and biological processes. Panel of genes such as WRKY and phenyl propanoid were found to be expressing during stresses in tomato. Evaluation of differentially expressed genes paves the way for candidate gene selection. These genes may be focussed in constructive breeding programs. Researchers recommend the reference tomato genome enrichment and bioinformatics workforce surge.


Keywords: Solanum lycopersicum, transcriptomics, tomato genome, RNA Seq., bioinformatics
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How to cite this article:

Kinjal Kulshrestha and Akarsh Parihar. 2020. A Decade of Tomato Transcriptomics: Status and Perspectives.Int.J.Curr.Microbiol.App.Sci. 9(3): 2026-2056. doi: https://doi.org/10.20546/ijcmas.2020.903.235