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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(3): 1850-1859
DOI: https://doi.org/10.20546/ijcmas.2020.903.214


Prevalence of Antimicrobial Resistance Patterns of Escherichia coli Faecal Isolates of Cattle
A. S. Mahmoud¹*, D. Mittal², G. Filia4, V. Ramneek³ and V. Mahajan4
¹Department of Preventive Medicine, Faculty Veterinary Medicine, University of Tripoli, Tripoli, Libya
²Department of Veterinary Public Health and Epidemiology, LalaLajpatRai University of Veterinary & Animal Sciences, Hisar-125004 (Haryana) India
³Department of Animal Biotechnology, School of Animal Biotechnology, GADVASU, Ludhiana
4Animal Disease Research Centre, GADVASU, Ludhiana, India, India
*Corresponding author
Abstract:

A total of 154 of Escherichia coli (E. coli) isolates were isolated from faecal specimens of healthy cattle (n=100). Serotyping of 154 E. coli isolates indicated that 112 (72.72%) isolates were typeable, 28 (18.18%) untypeable and 14 (9.09%) were rough. The most predominant serotype observed was O22 followed by O56, O60, O120 and O1. Antibiogram pattern using a total of 25 different antibiotics indicated that high resistant was present against clindamycin, metronidazole and penicillin followed by cephalothin, neomycin, kanamycin, cephalexin, streptomycin, furazolidone and tetracycline antibiotics. The amplification of tet(A) and tet(B) resistant determinants by PCR resulted in generation of 372bp and 228, respectively. The tet(A) gene was predominant gene compared to tet(B) gene. This study using pheno-genotypic characterization indicated the presence of antimicrobial resistant E. coliisolates amongst healthy cattle, emphasizing that effective strategy should be applied to persist the efficiency along with ideal usage of novel antibiotics though minimizing the risk of antibiotic resistant bacteria.


Keywords: E. coli, Antibiotics resistance, Multiplex PCR, tet genes, Risk factors
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How to cite this article:

Mahmoud, A. S., D. Mittal, G. Filia, V. Ramneek and Mahajan, V. 2020. Prevalence of Antimicrobial Resistance Patterns of Escherichia coli Faecal Isolates of Cattle.Int.J.Curr.Microbiol.App.Sci. 9(3): 1850-1859. doi: https://doi.org/10.20546/ijcmas.2020.903.214