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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(3): 473-481
DOI: https://doi.org/10.20546/ijcmas.2017.603.055


Molecular Characterization of Tomato Leaf Curl Virus (ToLCV) in South Gujarat, India
M. Vanthana1*, L. Mahatma1, T.V. Ghevariyaand R. Saranya2
1Department of Plant Pathology, NMCA, NAU, Navsari, Gujarat 396 450, India
2Department of Plant Pathology, JAU, Junagadh, Gujarat 362 001, India
*Corresponding author
Abstract:

The present study was conducted to characterize Tomato Leaf Curl Virus infecting tomato in south Gujarat region. Infected leaves showed typical leaf curl symptom with upward/downward curling along with vein clearing of the leaves. Using PCR a part of DNA-A molecule of ~1200 bp was amplified with a Begomovirus specific primers confirming it to be Begomovirus. On sequencing, a 1125 bp nucleotide sequence (Accession no- KU921251) was obtained. Amplified fragment had two genes viz., virus coat protein (V1) gene and pre coat protein (V2) gene. The sequenced virus showed highest identity (94%) with Tomato leaf curl Gujarat virus [Nepal] segment DNA A, [AY234383.1] followed by 93% sequence identity with thirteen different tomato Begomoviruses which were the strains/isolates of Tomato leaf curl Gujarat virus or Tomato leaf curl New Delhi virus [DQ629101.2]. The virus was named as Tomato leaf curl Gujarat virus [Nepal] [India:Nvs:Tom:2016] and was abbreviated as ToLCGV [Nepal] [India:Nvs:Tom:2016]. Phylogenetic analysis of the sequenced virus with the other Begomoviruses of the different crops from the different location indicated that the virus was typically a leaf curl virus.


Keywords: Begomovirus, DNA-A, ToLCV, PCR, Phylogeny
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How to cite this article:

Vanthana, M., L. Mahatma., T.V. Ghevariya and Saranya, R. 2017. Molecular Characterization of Tomato Leaf Curl Virus (ToLCV) In South Gujarat.Int.J.Curr.Microbiol.App.Sci. 6(3): 473-481. doi: https://doi.org/10.20546/ijcmas.2017.603.055