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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:3, March, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(3): 1388-1392
DOI: https://doi.org/10.20546/ijcmas.2020.903.161


Effect of Inoculation of Screened Rhizobial Isolates on the Growth of Pea (Pisum sativum (L.))
Srishty1, Anshu Sibbal Chatli2*, Irandeep Kaur1 and Parminder Kaur1
1Department of Biotechnology, Guru Nanak Girls College, Model Town, Ludhiana (Punjab), India
 2Department of Microbiology, Guru Nanak Girls College, Model Town, Ludhiana (Punjab), India
*Corresponding author
Abstract:

The present study was undertaken to obtain the best host cultivar-Rhizobial strain combination in the soils of Hoshiarpur. A Pot experiment was conducted by sowing seeds of Pea (Pisum sativum (L.)) varAP-3 along with screened isolated strains of Rhizobia in soil samples. After 60 days of sowing various parameters such as number of nodules, fresh weight and dry weight of plants, length of plants, leghaemoglobin content, nitrogenase activity and percent nitrogen content were recorded. Maximum number of nodules was observed in SS8 (53) followed by strain SS12 (20). The maximum fresh weight (11.35g/root and 12.20g/shoot), dry weight (8.17g/root and 9.16g/shoot)and length (20.3 cm/root and 17.0 cm/shoot)were reported in plants inoculated with SS8 strain of Rhizobia. The leghaemoglobin content in nodules was found to be highest in plants inoculated with SS8 strain (8.96 mg/0.5 g nodules) followed by plants inoculated with SS12 strain of Rhizobia (6.36 mg/0.5 g nodules). The nitrogenase activity (0.65 µM C2H2 reduced/plant) was also observed to be highest in plants inoculated with SS8 strain of Rhizobia. The total nitrogen content was 3.22% in SS8 inoculated plant leaves while it was 2.10% in SS12 inoculated plant leaves.


Keywords: Rhizobium, Pea (Pisum sativum (L.)), nitrogen-fixation, biofertilizers, plant growth

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How to cite this article:

Srishty, Anshu Sibbal Chatli, Irandeep Kaur and Parminder Kaur. 2020. Effect of Inoculation of Screened Rhizobial Isolates on the Growth of Pea (Pisum Sativum(L.)).Int.J.Curr.Microbiol.App.Sci. 9(3): 1388-1392. doi: https://doi.org/10.20546/ijcmas.2020.903.161
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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