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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(3): 1150-1158
DOI: https://doi.org/10.20546/ijcmas.2020.903.134


Effect of Substrate Concentration on Soil Enzyme Urease
Aruna Kumari1*, P.C.Rao2, G.Padmaja3and M. Madhavi4
1Department of Biochemistry, College of Agriculture, PJTSAU, Rajendranagar, Hyderabad, Telangana, India
 2Dean of Agricuilture (Retd.) PJTSAU, 3Department of SSAC, College of Agriculture, PJTSAU, India
4ACRPP, Weed control, Rajendranagar PJTSAU, India
*Corresponding author
Abstract:

To study the effect of substrate concentration on soil enzyme Urease in selected soils. Forty soil samples were assayed to measure the activity of the soil enzyme Urease among them four soil samples two Alfisols and two Vertisolssoils with high activity were selected for further study. Urease activities of the surface soils expressed as µg of NH4+ released g-1 soil h-1 ranged from 5.9 to 16.0 with an average value of 8.74.Soil enzyme Urease activity increased with an increase in substrate concentration in the beginning and almost reached a plateau at a substrate concentration of 30mM for all the four soils. With further increase in substrate concentration, minimal change in enzyme activity was observed. Characteristics of enzyme activities like maximum enzyme reaction velocity (Vmax) and Michaelis constant (Km) were determined using Michealis – Menten equation similar to those determined in homogenous system. The Km value range from 0.49mM to 0.60mM in Lineweaver - Burk Transformation and 0.50mM to 0.76mM in Hanes - Wolf Transformation and in case of Eadie - Hofstee Transformation the Km value range from 0.62mM to 0.78mM. Vertisols showed more km value than Alfisols. The Vmax value range from 8.1µg of NH4+ released g-1 soil h-1to 10 µgof NH4+ released g-1 soil h-1 in Lineweaver - Burk Transformation and 8.7(µg of NH4+ released g-1 soil h-1  to 10.3 (µg of NH4+ released g-1 soil h-1 in Hanes - Wolf Transformation and in case of Eadie - Hofstee Transformation the  max range from 9.1(µg of NH4+ released g-1 soil h-1 to 10.5 (µg of NH4+ released g-1 soil h-1 . Vmax value range from 8.1 (µg of NH4+ released g-1 soil h-1to 9.7(µg of NH4+ released g-1 soil h-1 in Vertisols and 9.5 (µg of NH4+ released g-1 soil h-1  to 10.5 (µg of NH4+ released g-1 soil h-1 in Alfisols and Alfisols showed more Vmax value than Vertisols


Keywords: Alfisols,Eadie - Hofstee Transformation,Hanes - Wolf Transformation, Lineweaver - Burk Transformation,
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How to cite this article:

Aruna Kumari, J., P.C.Rao, G.Padmaja and Madhavi, M.2020. Effect of Substrate Concentration on Soil Enzyme Urease. Int.J.Curr.Microbiol.App.Sci. 9(3): 1150-1158. doi: https://doi.org/10.20546/ijcmas.2020.903.134