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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(3): 831-835
DOI: https://doi.org/10.20546/ijcmas.2020.903.097


In vitro Sterilization Protocol for Establishment of  Litchi (Litchi chinensis Sonn) cv. Purbi
Neha Nischal, Hidayatullah Mir, Shaheena Parveen*, Shashi Prakash,
Ruby Rani and Sanjay Sahay
Department of Horticulture (Fruit & Fruit Technology), Bihar Agricultural University, Bhagalpur, 813210, Bihar, India
*Corresponding author
Abstract:

Litchi is considered a crop difficult to propagate through micropropagation and obtaining contamination free cultures is first and foremost requirement of tissue culture. So the present investigation was carried out to standardize the sterilization procedure of nodal segment and leaf explants of Litchi chinensis Sonn. cv. Purbi. Two surface sterilizing agents viz. HgCl2 and NaOCl were used at varying concentrations and durations. In this experiment HgCl2 was found to be better sterilizing agent than NaOCl for both the explants. HgCl2 (0.2 %) for 3 min treatment was found to be the most effective for nodal segment explants that resulted in maximum per cent survival (56.5±1.19%), low percent mortality (13.8±0.18%) and low per cent contamination (33.1±0.51%). While for leaf explants HgCl2 (0.1 %) for 1.0 min yielded best results with highest per cent survival (53.9±1.08), low per cent mortality (14.0±0.21) and low per cent contamination (35.5±0.56).


Keywords: Litchi, Micropropagation, NaOCl, HgCl2, Mortality
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How to cite this article:

Neha Nischal, Hidayatullah Mir, Shaheena Parveen, Shashi Prakash, Ruby Rani and Sanjay Sahay. 2020. In vitro Sterilization Protocol for Establishment of Litchi (Litchi chinensis Sonn) cv. Purbi. Int.J.Curr.Microbiol.App.Sci. 9(3): 831-835. doi: https://doi.org/10.20546/ijcmas.2020.903.097