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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(2): 3124-3217
DOI: https://doi.org/10.20546/ijcmas.2020.902.361


Genetic Variability of Rice Phytoplasma by using Heteroduplex Mobility Assay (HMA)
P. Valarmathi1* and D. Ladhalakshmi2
1ICAR-Central Institute for Cotton Research (CICR), Coimbatore-641 003, India 2Department of Plant Pathology, ICAR-Indian Institute of Rice Research (IIRR), Hyderabad-500 030, India
*Corresponding author
Abstract:

Heteroduplex mobility assay (HMA) is based on the principle that DNA heteroduplexes formed between related sequences have a reduced mobility in polyacrylamide gels proportional to their degree of divergence. HMA has been reported as a reliable means for identification and classification of phytoplasmas. Recently, the 16/23S spacer region has been demonstrated to be the best choice, when using the heteroduplex mobility assay (HMA) to differentiate closely related phytoplasmas and to group field isolates into well characterized phytoplasmas. The rice samples of varieties BPT 5204, White Ponni, CO 39 and ADT 43 produced no heteroduplex with assay, signifying that they are very closely related to each other.


Keywords: Candidatus Phytoplasma, mycoplasma-like organisms, heteroduplex
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How to cite this article:

Valarmathi, P. and Ladhalakshmi, D. 2020. Genetic Variability of Rice Phytoplasma by using Heteroduplex Mobility Assay (HMA).Int.J.Curr.Microbiol.App.Sci. 9(2): 3124-3217. doi: https://doi.org/10.20546/ijcmas.2020.902.361