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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(2): 3080-3087
DOI: https://doi.org/10.20546/ijcmas.2020.902.355


Assessment of Various Sterilants on Differential Growth Response in Carica papaya
Nisha Malik1*, Rakesh Singh Sengar1, Devendra Kumar1, Shiv Kumar Singh2, Manoj Kumar Yadav1, Mukesh Kumar3 and Gopal Singh4
1Department of Agricultural Biotechnology, College of Agriculture, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut -250110 (U.P.), India 2Department of Genetics and Plant Breeding, College of Agriculture, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut -250110 (U.P.), India 3College of Horticulture, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut -250110 (U.P.), India 4Department of Plant Pathology, College of Agriculture, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut -250110 (U.P.), India
*Corresponding author
Abstract:

Papaya is a nutraceutical fruit crop grown among tropical and sub-tropical regions of the world. The propagation through seeds results in the production of non-true-type planting materials due to segregation of off-springs at second filial generation leading to inherent heterozygosity. The alternative strategy to this is in-vitro propagation, but the main hurdles comes under this method is failure to culture establishment. Thus, searching for an effective sterilization is always needed. Therefore, present study was intended to evaluate the effect of various sterilant on culture establishment. The lateral buds were collected as explant and treated with 0.1% Bavistin, 0.1% HgCl2, 4% NaOCl and 70% EtOH for different time interval. The higher survival percent and uniform growth pattern was observed with 0.1% Bavistin for 10 min (38.7), 0.1% HgCl2 for 2 min (40.5), 4% NaOCl (39) and 70% EtOH for 1 min (37.8), whereas combination of these sterilant showed 46.3% shoot survival with uniform growth.


Keywords:
Sterilant, Contamination, Papaya, Survival
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How to cite this article:

Nisha Malik, Rakesh Singh Sengar, Devendra Kumar, Shiv Kumar Singh, Manoj Kumar Yadav, Mukesh Kumar and Gopal Singh. 2020. Assessment of Various Sterilants on Differential Growth Response in Carica papaya.Int.J.Curr.Microbiol.App.Sci. 9(2): 3080-3087. doi: https://doi.org/10.20546/ijcmas.2020.902.355