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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(3): 389-395
DOI: https://doi.org/10.20546/ijcmas.2020.903.046


Detection of Bhendi Yellow Vein Mosaic Virus (BYVMV) from the Different Parts of Bhendi [Abelmoschus esculentus (L.) Moench] Plant, Flower and Seed
Priyal Sisodia and Lalit Mahatma*
Department of Plant Pathology, N.M. College of Agriculture, Navsari Agricultural University, Navsari 396 450 Gujarat (India)
*Corresponding author
Abstract:

During the present investigation yellow vein mosaic disease in bhendi (Abelmoschus esculentus (L.) Moench) caused by a whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) transmitted Begomovirus particularly Bhendi yellow vein mosaic virus [IN-NVS-2018] or BYMV [IN-NVS-2018] or BYMV was characterized by symptoms of homogenous interwoven network of yellow veins enclosing islands of green tissues. In extreme cases, the infected leaf becomes totally light yellow or cream colored. The fruits of the infected plants exhibit pale yellow color, become deformed, small and tough in texture. Modification of the Doyle and Doyle (1987) method for the DNA extraction in which instead of 750µl each CTAB buffer and Chloroform: Isoamyl Alcohol (1:24), 1000µl each pre-heated CTAB extraction buffer Chloroform: Isoamyl Alcohol (1:24) was used for the proper precipitation of proteins and carbohydrates and yielded good quality of DNA. The presence of BYMV was detected from all the part tested viz., tip of the stem, tip of the root, flower bud, fruit, petel, sepal, ovary, pollen, seed coat, cotyledon and embryonic axis. Results indicated that the virus is present in all the parts of the plants including, vegetative and reproductive parts of the flowers as well as in seeds. However, in grow out test, none of the sample showed symptoms on the seedling. Therefore, it can be concluded that the virus is present in the seed, however, is not seed transmitted. From the best of our knowledge, this is the first report in the world which sowed the presence of BYVMV in bhindi seeds.


Keywords: Begomovirus, BYVMV, Okra, Bhendi, Seed borne infection
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How to cite this article:

Priyal Sisodia and Lalit Mahatma. 2020. Detection of Bhendi Yellow Vein Mosaic Virus (BYVMV) from the Different Parts of Bhendi (Abelmoschus esculentus (L.) Moench) Plant, Flower and Seed.Int.J.Curr.Microbiol.App.Sci. 9(3): 389-395. doi: https://doi.org/10.20546/ijcmas.2020.903.046