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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(3): 319-327
DOI: https://doi.org/10.20546/ijcmas.2020.903.038


Isolation and Molecular Identification of Riemerella anatipestifer from Ducks in Assam
Ritam Hazarika1*, M. K. Doley2, H. Sarmah2, P. Deka2 and R. K. Sharma2
1Department of Animal Biotechnology
2Department of Microbiology, College of Veterinary Science, A.A.U. Khanapara, Guwahati-781022, Assam, India
*Corresponding author
Abstract:

Considering the highest duck population in the eastern part of India, a preliminary study was carried out in Assam to investigate duck septicaemia like disease condition and the association of Riemerella anatipestifer in ducks. Among the 98samples collected from ducks (34) comprising healthy ducks (10), ducks with clinical symptoms (19), suggestive of duck septicaemia and dead ducks (5); 13 samples revealed isolation of R. anatipestifer. Molecular confirmation of the isolates, using R. anatipestifer specific PCR assay (564 bp), as well as gyrB-based PCR (162 bp) revealed both the genes to be suitable molecular markers for identification of the isolates as R. anatipestifer. Kirby-Bauer disc diffusion tests were used to analyze the antibiotic resistance of 13R. anatipestifer isolates. The present study is the first report of molecular detection of R. anatipestifer from ducks in Guwahati, Assam and suggests that the PCR assay can facilitate fast and accurate identification of R. anatipestifer infection in ducks during disease outbreaks.


Keywords: Assam, Ducks, Polymerase Chain Reaction, Riemerella anatipestifer
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How to cite this article:

Ritam Hazarika, M. K. Doley, H. Sarmah, P. Deka and Sharma, R. K. 2020. Isolation and Molecular Identification of Riemerella anatipestifer from Ducks in Assam.Int.J.Curr.Microbiol.App.Sci. 9(3): 319-327. doi: https://doi.org/10.20546/ijcmas.2020.903.038