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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(3): 308-312
DOI: https://doi.org/10.20546/ijcmas.2020.903.036


Diagnostic Role of Polymerase Chain Reaction and Inadequacy of Mycobacterium Growth Indicator Tube culture in Tuberculous Pleuritis
Gandhi Kandhakumari1* and Selvaraj Stephen2
1Kanti Devi Medical College and Research Centre, Mathura, Uttar Pradesh-281406, India
2Department of Microbiology, Mahatma Gandhi Medical College & Research Institute, SBV University, Pillaiyarkuppam, Pondicherry - 607403, India
*Corresponding author
Abstract:

Diagnosis and management of patients with tuberculous pleuritis needs a rapid and sensitive diagnostic tool. Our aim was to diagnose cases of tuberculous pleuritis from patients employing ZN smear, culture (LJ & MGIT) and to compare with PCR. Pleural fluid collected from 36 patients with clinical and/or radiological suspicion of TB pleuritis was included in this study. Pleural fluid collected from nine non-tuberculous pleuritis cases was included as negative controls. Pleural fluid samples were analyzed for ZN smear, LJ and automated MGIT culture and results compared with MTB PCR targeting IS6110 region. Positivity for smear, LJ, MGIT and PCR individually were 5.6%, 2.8%, 16.7% and 25% respectively. Combination of all above techniques together increased the percentage positivity to 30.6%. Negative controls were negative by all procedures. In the context of paucibacillary specimens like pleural fluid, smear microscopy and LJ are less sensitive. MGIT has moderate sensitivity and rapidity compared to LJ. PCR is rapid and highly sensitive, but needs interpretation of PCR reports with “gold standard” culture and clinical history to arrive at an early and accurate diagnosis.


Keywords: PCR, Mycobacterium tuberculosis, Pleural fluid, LJ, IS6110
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How to cite this article:

Gandhi Kandhakumari and Selvaraj Stephen. 2020. Diagnostic Role of Polymerase Chain Reaction and Inadequacy of Mycobacterium Growth Indicator Tube culture in Tuberculous Pleuritis.Int.J.Curr.Microbiol.App.Sci. 9(3): 308-312. doi: https://doi.org/10.20546/ijcmas.2020.903.036