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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(3): 243-248
DOI: https://doi.org/10.20546/ijcmas.2020.903.029


Effect of Culture Media on Shoot Proliferation and Callus Induction of Bael (Aegle marmelos L.)
Pukh Raj, Mohan Lal Jakhar, Sarfraz Ahmad*, Kassim Yahya Mtilimbanya, Suman Chahar and Hari Ram Jat
Department of Plant Breeding and Ge0netics, S.K.N. Agriculture University, Jobner, Rajasthan-303029, India
*Corresponding author
Abstract:

The present investigation was carried out at Tissue Culture Laboratory of S.K.N. Agriculture University, Jobner, India during 2018-19. Experiment was laid out using completely randomized design (CRD) with ten replications. Six culture media viz. Murashige and Skoog media, Nitsch & Nitsch media, woody plant medium, Schenk and Hildebrant medium, White’s medium and Khudson solution–C were tested for direct shoot proliferation and callus induction at most responsive level of plant growth regulators in Bael (Aegle marmelos L.). Highest shoot bud induction (4.1) was observed using woody plant medium with 100 per cent morphogenetic response using nodal segment explant along with BAP at 2 mg/l concentration. Maximum callus induction on leaf explant was observed on Murashige and Skoog medium with 40 per cent morphogenetic response followed by woody plant medium (30 per cent) with 2,4-D at 2 mg/l concentration. Thus for large scale micropropagation, woody plant medium using nodal segment explant is recommended for shoot proliferation and for callus induction, MS medium taking leaf as explant will be rewarding for mass multiplication of Bael.


Keywords: Aegle marmelos, Callus induction, Micropropagation, Murashige and Skoog medium, shoot proliferation, Woody Plant Medium.
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How to cite this article:

Pukh Raj, Mohan Lal Jakhar, Sarfraz Ahmad, Kassim Yahya Mtilimbanya, Suman Chahar and Hari Ram Jat. 2020. Effect of Culture Media on Shoot Proliferation and Callus Induction of Bael (Aegle marmelos L.).Int.J.Curr.Microbiol.App.Sci. 9(3): 243-248. doi: https://doi.org/10.20546/ijcmas.2020.903.029