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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(2): 2942-2947
DOI: https://doi.org/10.20546/ijcmas.2020.902.335


Qualitative Toxicity Bioassay of Bacteria Photorhabdus Associated Symbiotically with Entomopathogenic Nematode Heterorhabditis against Galleria mellonella
Amit Ahuja1*, Ajay Singh Sindhuand Jagdish Yadav2
 1Division of Nematology, ICAR-Indian Agriculture Research Institute, New Delhi, India
2Division of Plant Pathology, ICAR-Indian Agriculture Research Institute, New Delhi, India
*Corresponding author
Abstract:

The bacteria Photorhabdus associated symbiotically with the entomopathogenic nematodes of genus Heterorhabditis is an effective killer of insect-pests belonging to a different order. The bacteria Photorhabdus encodes numerous toxins once inside the midgut of insects and cause rapid death by employing multiple toxicity mechanism. In this paper, we isolated the bacteria Photorhabdus associated with Heterorhabditis nematodes using a modified White trap and demonstrated a bioassay of toxicity against last instar larva of wax moth Galleria mellonella. The larva was injected with 25µl bacterial suspension inside the midgut by micro syringe at undiluted and diluted concentration. The bacteria was found quick in killing the larva with a medium lethal time 12hr post-injection at both of the concentration. The dead larva turned brick-red upon infestation by bacteria Photorhabdus. The bacteria perform three major tasks once inside the insect body, which includes the production of the digestive enzyme to convert the insect’s tissue into a suitable growth medium. Later bacteria encode multiple toxin protein to kills it hosts and finally release several secondary antibiotic compounds to prevent the growth of any other microbe on the dead cadaver. The full potential of bacteria in crop protection schemes against insect-pests of crops is yet to be realized. The bacteria Photorhabdus have the full potential to be utilized as an alternative of already existing Bt crops once the biological pathways and genes involved in toxicity are understood.


Keywords: Entomopathogenic nematode, Heterorhabditis, Photorhabdus, Galleria mellonella, Bioassay
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How to cite this article:

Amit Ahuja, Ajay Singh Sindhu and Jagdish Yadav. 2020. Qualitative Toxicity Bioassay of Bacteria Photorhabdus Associated Symbiotically with Entomopathogenic Nematode Heterorhabditis against Galleria mellonella.Int.J.Curr.Microbiol.App.Sci. 9(2): 2942-2947. doi: https://doi.org/10.20546/ijcmas.2020.902.335