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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(2): 2929-2934
DOI: https://doi.org/10.20546/ijcmas.2020.902.333


Fluorescence Activated Cell Sorting (FACS) of CK15 Cells in Canine Hair Follicular Tissue
N. Hemavathi1, Sabiha Hayath Basha2*, S. Usha Kumary1 and R. Sivashankar3
1Department of Veterinary Anatomy, Madras Veterinary College, TANUVAS, Chennai-600 007, Tamil Nadu, India
2*Centre for Stem Cell Research and Regenerative Medicine, Madras Veterinary College, TANUVAS, Chennai-600 007, Tamil Nadu, India
3Department of Veterinary Surgery and Radiology, Madras Veterinary College, TANUVAS, Chennai-600 007, Tamil Nadu, India
*Corresponding author
Abstract:

Hair follicle is an ideal system for studying the biology and regulation of adult stem cells. Multipotent stemness of the canine hair follicle stem cells (cHFSCs) were detected by immunostaining the cells with positive HFSC surface markers like Anti-cytokeratin-15 (CK15) and CD200. In this study, canine hair follicular cells were cultured in Williams’E media supplemented with growth factors. The primary cultures of the hair follicles were subjected to Fluorescence Activated Cell Sorting (FACS) and then cells were immunostained with CK15 and DAPI. In primary culture, 18.28% of cells were positive for CK15 and sorted out by FACS. The positive expression of CK15 transcription factor was observed with DAPI as a counter stain in the in-vitro cultured cHFSCs. These results indicated that the cells in the canine hair follicular bulge region possess the stemness and act as a precursor and involved in hair cycle.


Keywords: cHFSCs, CK15, FACS, Stem cells,
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How to cite this article:

Hemavathi. N, Sabiha Hayath Basha, S. Usha Kumary and Sivashankar. R. 2020. Fluorescence Activated Cell Sorting (FACS) of CK15 Cells in Canine Hair Follicular Tissue.Int.J.Curr.Microbiol.App.Sci. 9(2): 2929-2934. doi: https://doi.org/10.20546/ijcmas.2020.902.333