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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(2): 2752-2760
DOI: https://doi.org/10.20546/ijcmas.2020.902.313


Assessment of Genetic Diversity and to Study the Relationship in Selected Green Gram Germplasm by ISSR Marker
S. S. Mangave1*, N. B. Gokhale1, C. B. Kuchekar2, S. V. Sawardekar1 and J. P. Devmore3
1Plant Biotechnology Centre, College of Agriculture, D.B.S.K.K.V. Dapoli, India
2J.B. Krishna College of Agriculture, RethareBk, India
3DepartmentofAgriculturalBotany, College of Agriculture, D.B.S.K.K.V. Dapoli, India
*Corresponding author
Abstract:

The molecular marker technology has a great potential for assessing genetic diversity and relationship among the selected varieties. In the present study 52 germplasms of green gram showing distinct morphological differences were screened using 15 ISSR markers. The DNA was extracted from the green leaf samples collected. Modification in extraction procedure resulted into better and clear banding pattern when subjected to PCR analysis. Modification in PCR parameters like PCR master mixture and thermo profile showed clear and specific banding pattern. The green gram DNA showed better amplification with 15 ISSR primers. Total 2721 bands were amplified and out of which 2671 were polymorphic which showed 92.23 % polymorphism. The primer UBC-814 do not showed polymorphism. The size of amplified fragment ranged from 300bp to 2000bp. The PIC value ranges from 0.00 to 0.87 and average polymorphic information content is 0.75. It indicates that ISSR markers have a great potential to show the polymorphism among the green gram germplasms. The data were used to generate pair-wise matrix based on the Jaccard’s Similarity co-efficient. The genetic distance was calculated on the basis of pooled data and the dendrogram was constructed. The similarity co-efficient ranged from 0.093 to 0.596 indicating the distinctness and similarities of these germplasms.


Keywords: ISSR, Green gram,Vignaradiata,Molecular marker, genetic diversity.
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How to cite this article:

Mangave. S. S, N. B. Gokhale, C. B. Kuchekar, S. V. Sawardekar and Devmore. J. P. 2020. Assessment of Genetic Diversity and to Study the Relationship in Selected Green Gram Germplasm by Issr Marker.Int.J.Curr.Microbiol.App.Sci. 9(2): 2752-2760. doi: https://doi.org/10.20546/ijcmas.2020.902.313