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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(2): 2254-2261
DOI: https://doi.org/10.20546/ijcmas.2020.902.255


Isolation and Characterization of Plant Growth Promoting Rhizobacteria from Northern Karnataka, India
M. Chiranjeevi1*, Geeta Goudar2, P. U. Krishnaraj2 and M. M. Jamadar3
1Department of Agricultural Microbiology, College of Agriculture, Vijayapur, India
2Department of Agricultural Microbiology, College of Agriculture, Dharwad, India
3Department of Plant Pathology, College of Agriculture, Vijayapur University of Agricultural Sciences, Dharwad-580005, Karnataka, India
*Corresponding author
Abstract:

An attempt was made to isolate and characterize rhizobacteria from rhizosphere soils of different crops grown in Vijayapur, Kalaburgi and Bagalkot districts of Northern Karnataka. Ten PGPR isolates and reference strain were subjected for functional characterization viz., IAA and GA production, P and Zn solubilization. Among the ten native rhizobacterial isolates, 8 isolates and reference strain produced IAA and GA. The amount of IAA and GA produced by the different rhizobacterial isolates ranged from 10.78 to 20.17 µg/ml of broth and 0.36 to 2.69 µg/ml of broth respectively. The diameter of P and Zn solubilization by the rhizobacterial isolates ranged from 3 to 7.5 mm and 2.0 to 6.0 mm after five days of inoculation respectively. These isolates were further subjected for morphological and biochemical characterization. The isolates exhibited wide variations with respect to colony morphology. Except one isolate (PG-189), other isolates found to have rod shaped cells. Except PG-152 and PG-186, other isolates and reference strain were found to be gram negative. Endospore formation was observed only in the isolate PG-152. With respect to biochemical characterization, all the isolates and reference strain were positive for oxidase and ammonia production tests and negative for indole production and lactose fermentation.


Keywords: PGPR, IAA, GA, P-solubilizers
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How to cite this article:

Chiranjeevi, M., Geeta Goudar, P. U. Krishnaraj and Jamadar, M. M. 2020. Isolation and Characterization of Plant Growth Promoting Rhizobacteria from Northern Karnataka, India.Int.J.Curr.Microbiol.App.Sci. 9(2): 2254-2261. doi: https://doi.org/10.20546/ijcmas.2020.902.255