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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(2): 2132-2140
DOI: https://doi.org/10.20546/ijcmas.2020.902.242


Conservation and In Vitro Propagation of an Endangered Wild Turmeric (Curcuma caesia Roxb.) Species from Sub - Himalayan Terai Region of West Bengal
Sayan Chowdhury1, Kumaresh Pal1, Moumita Chakraborty2, Soumendra Chakraborty2, Somnath Mandal1, Goutam Kr. Pandit1, Soumen Maitra3 and Nandita Sahana1*
1Department of Biochemistry, Uttar Banga Krishi Viswavidyalaya, Pundibari-736165, Cooch Behar, West Bengal, India
2Department of Genetics and Plant Breeding, Uttar Banga Krishi Viswavidyalaya, Pundibari-736165, Cooch Behar, West Bengal, India
3Department of Floriculture, Medicinal and Aromatic Plants, Uttar Banga Krishi Viswavidyalaya, Pundibari-736165, Cooch Behar, West Bengal, India
*Corresponding author
Abstract:

Curcuma caesia Roxb. is a perennial herb with bluish-black rhizome and reportedly harbors medicinal properties. The powder and essential oil of the rhizome of the herb has potential use in pharmaceutical and cosmetic industries. This important wild turmeric species has also been declared as endangered. Conservation and propagation of the black turmeric species by in vitro techniques is of high importance. The present study was conducted to develop an efficient protocol for mass propagation and conservation of Curcuma caesia collected from northern part of West Bengal. Bud from rhizomes as explants was cultured on full strength Murashige and Skoog (MS) medium for 19 days. For multiplication purpose, the explants were transferred either in MS medium supplemented with 6-Benzylaminopurine (BAP) and IAA or Kinetin (KIN) with BAP for 56 days. MS medium supplemented with a combination of BAP 2.0 mg/L and IAA 0.75 mg/L gave the highest number (four) of shoots. MS medium supplemented with a combination of 2.0 mg/L IBA gave the highest number (twelve) of roots after 28 days. Healthy and complete plants with well-developed roots were hardened, acclimatized and planted in the field successfully with a survival rate of 70%. 2.0 mg/L 2,4-D showed the best result of callus induction for 83 days.


Keywords: Curcuma caesia, micro-propagation, explant, in vitro, BAP, IAA, IBA, Kinetin, 2, 4-D
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How to cite this article:

Sayan Chowdhury, Kumaresh Pal, Moumita Chakraborty, Soumendra Chakraborty, Somnath Mandal, Goutam Kr. Pandit, Soumen Maitra and Nandita Sahana. 2020. Conservation and In Vitro Propagation of an Endangered Wild Turmeric (Curcuma Caesia Roxb.) Species from Sub - Himalayan Terai Region of West Bengal.Int.J.Curr.Microbiol.App.Sci. 9(2): 2132-2140. doi: https://doi.org/10.20546/ijcmas.2020.902.242