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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(2): 2059-2071
DOI: https://doi.org/10.20546/ijcmas.2020.902.234


The Effect of Different Supplements to in vitro Maturation Medium on Nuclear Maturation Rate of Buffalo Oocytes Assessed based on Stage of Nucleus
Ambadas1*, G. Sarvadnya2, K. Shrikant3 and K. B. Satish1
1Department of Veterinary Physiology and Biochemistry, Veterinary College, Bidar, India
2Veterinary Dispensary Donagapur Tq Bhalki Dist, Bidar, India
3Department of Veterinary Physiology and Biochemistry, Veterinary College, Bidar, India
*Corresponding author
Abstract:

For comparing the efficacy of serum gonadotropin @50 and 100IU/ml, estrodiol-17β @0.5 and 1μg/ml, EBS @5 and 10% and BFF @5 and 10% supplementation on in vitro nuclear maturation rate of buffalo oocytes. Ovaries were collected from buffaloes slaughtered at local abattoir. Oocytes were aspirated and good and excellent quality oocytes were matured in basic maturation media TCM199 supplementing above mentioned supplements separately. The recovery rate of culturable oocytes was 85.33%. The nuclear maturation rate (NMR) was highest in the oocytes supplemented with 10% EBS and lowest with estradiol-17β @1µg/ml. The statistical analysis revealed that NMR was significantly (p≤0.05) higher in the group supplemented with 10% EBS as compared to all other groups. It can be concluded that the supplementation of EBS to IVM medium has positive effect on nuclear maturation rate. Overall results suggest that serum gonadotrophin is less effective than EBS but more effective than estradiol-17β and BFF. Buffalo follicular fluid is more effective than estradiol-17β but less effective than EBS and serum gonodotrophin. Estrodiol-17β is the least effective supplement to increase in vitro nuclear maturation rate of buffalo oocytes.


Keywords: Maturation medium, Nuclear maturation rate, Buffalo oocytes
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How to cite this article:

Ambadas, G. Sarvadnya, K. Shrikant and Satish, K. B. 2020. The Effect of Different Supplements to in vitro Maturation Medium on Nuclear Maturation Rate of Buffalo Oocytes Assessed based on Stage of Nucleus.Int.J.Curr.Microbiol.App.Sci. 9(2): 2059-2071. doi: https://doi.org/10.20546/ijcmas.2020.902.234