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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(2): 1919-1931
DOI: https://doi.org/10.20546/ijcmas.2020.902.219


Comparison of the Efficacy of Serum Gonadotrophin, Estrodiol-17β, Estrous Buffalo Serum and Buffalo Follicular Fluid Supplementation on invitro Cytoplasmic Maturation Rate of Buffalo Oocytes
Ambadas1*, K. Shrikant1, G. Sarvadnya2 and K. B. Satish1
1Deptartment of Veterinary Physiology and Biochemistry, Veterinary College, Bidar, India
2Veterinary Dispensary, Donagapur Tq, Bhalki Dist, Bidar, India
*Corresponding author
Abstract:

An experiment was conducted to compare the efficacy of serum gonadotropin @50 and 100IU/ml, estrodiol-17β @0.5 and 1μg/ml, EBS @5 and 10% and BFF @5 and 10% supplementation on in vitro cytoplasmic maturation rate of buffalo oocytes. Ovaries were collected from buffaloes slaughtered at local abattoir. Oocytes were aspirated and good and excellent quality oocytes were matured in basic maturation media TCM199 supplementing above mentioned supplements separately. The recovery rate of culturable oocytes was 85.33%. The cytoplasmic maturation rate (CMR) was highest in the oocytes supplemented with 10% EBS and lowest with estradiol-17β @1µg/ml. The statistical analysis revealed that CMR was significantly (p≤0.05) higher in the groups supplemented with 5% EBS and 10% EBS as compared to others. It can be concluded that the supplementation of EBS to IVM medium has positive effect on cytoplasmic maturation rate. Overall results suggest that serum gonadotrophin is less effective than EBS but more effective than estradiol-17β and BFF. Buffalo follicular fluid is more effective than estradiol-17β but less effective than EBS and serum gonodotrophin. Estrodiol-17β is the least effective supplement to increase in vitro cytoplasmic maturation rate of buffalo oocytes.


Keywords: In vitro – Inside the Controlled Environment. Oocyte - Cell Of an Ovary
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How to cite this article:

Ambadas, K. Shrikant, G. Sarvadnya and Satish. K. B. 2020. Comparison of the Efficacy of Serum Gonadotrophin, Estrodiol-17β, Estrous Buffalo Serum and Buffalo Follicular Fluid Supplementation on In Vitro Cytoplasmic Maturation Rate of Buffalo Oocytes.Int.J.Curr.Microbiol.App.Sci. 9(2): 1919-1931. doi: https://doi.org/10.20546/ijcmas.2020.902.219