National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
An experiment was conducted to compare the efficacy of serum gonadotropin @50 and 100IU/ml, estrodiol-17β @0.5 and 1μg/ml, EBS @5 and 10% and BFF @5 and 10% supplementation on in vitro cytoplasmic maturation rate of buffalo oocytes. Ovaries were collected from buffaloes slaughtered at local abattoir. Oocytes were aspirated and good and excellent quality oocytes were matured in basic maturation media TCM199 supplementing above mentioned supplements separately. The recovery rate of culturable oocytes was 85.33%. The cytoplasmic maturation rate (CMR) was highest in the oocytes supplemented with 10% EBS and lowest with estradiol-17β @1µg/ml. The statistical analysis revealed that CMR was significantly (p≤0.05) higher in the groups supplemented with 5% EBS and 10% EBS as compared to others. It can be concluded that the supplementation of EBS to IVM medium has positive effect on cytoplasmic maturation rate. Overall results suggest that serum gonadotrophin is less effective than EBS but more effective than estradiol-17β and BFF. Buffalo follicular fluid is more effective than estradiol-17β but less effective than EBS and serum gonodotrophin. Estrodiol-17β is the least effective supplement to increase in vitro cytoplasmic maturation rate of buffalo oocytes.
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