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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(2): 1589-1603
DOI: https://doi.org/10.20546/ijcmas.2020.902.183


Phytosensitization and Cytotoxic Studies of Anacardium occidentale L. on Cancer Cell Lines – A Herbaceutical Approach
Amar Shankar1, S. M. Gopinath2* and M. Ismail Shareef1
1Department of Biotechnology, Acharya Institute of Technology, Acharya Doctor Sarvepalli Radhakrishnan Rd, Soladevanahalli, Karnataka, India- 560107
2Department of Biotechnology, Davangere University, Shivagangotri, Davangere, Karnataka, India- 577002 
*Corresponding author
Abstract:

Phytobioactives have been proved to be effective on various ailments and diseases. The same logic is been used by many traditional healers and practitioners and is evident from ancient scripts of Ayurveda. One such, less explored plant is Anacardium occidentale L., (Anacardiaceae). The phenolics from the leaves and bark have been partially explored in folklore medicine but the probable mode of action is still unclear. Hence the current study aims at the exploration of the anti-cancer potency of phytobioactives from Anacardium occidentale. Qualitative phytochemical profiling of the leaves and bark, followed by polarity based hot extraction procedure (hexane, ethyl acetate, methanol), In vitro antioxidant studies (ABTS, nitric oxide, reducing power radical assays) and cytotoxic studies were performed on six cell lines (Vero, L929, A549, MCF 7, U87 and Hela). The probable mode of action on p38α MAP kinase was proposed based on In silico molecular docking structure-activity relationship (SAR) studies wherein partially purified fractions predominately contained zoapatanolide A (diterpenoid), a gas this flav one (biflavonoid) and anacardic acid (phenolic acid) were considered, out of which a gas this flav one had an atomic contact energy (ACE) values of -345.06 than compared to the standard drug doxorubicin (-409.14). The results of the current study inferred the potency of the herbal cocktail as antioxidant and being functional against cancer cell lines (ethyl acetate fraction). Further, studies could be extrapolated for the purification of the active ingredients from Anacardium occidentale in search for potent, newer therapeutics.


Keywords: Anacardium occidentale, Anti-cancer, Antioxidant, Cytotoxic, In vitro, In silico, MAP kinase
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How to cite this article:

Amar Shankar, S. M. Gopinath and Ismail Shareef, M. 2020. Phytosensitization and Cytotoxic Studies of Anacardium occidentale L. on Cancer Cell Lines – A Herbaceutical Approach. Int.J.Curr.Microbiol.App.Sci. 9(2): 1589-1603. doi: https://doi.org/10.20546/ijcmas.2020.902.183