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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(2): 1575-1582
DOI: https://doi.org/10.20546/ijcmas.2020.902.181


Effect of Fungal Extracts on Bacillus thuringiensis Serotypes Causing Flacherie Disease in Mulberry Silkworm, Bombyx mori L.
Saravana Kumar1*, P. Sudhakara Rao1*, K. M. Vijaya Kumari1 and R. K. Mishra2
1Silkworm Seed Technology Laboratory, Central Silk Board, Kodathi,
Bangalore-500 035, India
2National Silkworm Seed Organization, Central Silk Board, Banglore-560068, India
*Corresponding author
Abstract:

Antibacterial activity of six different species of fungal extracts viz., Aspergillus ochraceous, Paecilomyces variotii, Schizophyllum sp., Epicoccum sp., Metarhizium sp., Aspergillus niger were evaluated against ten serotypes of Bacillus thuringiensis to develop new bioactive secondary metabolites from the microbial sources. Fungal crude extracts were isolated by ethyl acetate solvent extraction method and product yields varied from 0.150g to 0.538g.  Thin Layer Chromatography was carried out for detecting the small molecule compounds present in the crude extracts.  Antibacterial activities of fungal extracts were analyzed by disc diffusion method. All ten B.t serotypes were inhibited by six fungal extracts with zone of inhibition ranging from 12 ± 1.73 to 36 ± 1.53. Among the six extracts Paecilomyces variotii showed highest inhibition of 36 mm against B.t sotto. In vitro and in vivo studies conducted for the effect of extracts with different concentration and exposure duration reveals that effective against all serotypes. The results suggested that the Microbial derived extracts could be a new source of alternative in developing an effective bioactive molecule for the management of silkworm bacterial diseases.


Keywords: Silkworm, Flacherie, Bacillus thuringiensis, Fungal extract, Metabolite, Antibacterial activity
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How to cite this article:

Saravana Kumar, R., P. Sudhakara Rao, K. M. Vijaya Kumari and Mishr, R. K. 2020. Effect of Fungal Extracts on Bacillus thuringiensis Serotypes Causing Flacherie Disease in Mulberry Silkworm, Bombyx mori L..Int.J.Curr.Microbiol.App.Sci. 9(2): 1575-1582. doi: https://doi.org/10.20546/ijcmas.2020.902.181