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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(2): 1566-1574
DOI: https://doi.org/10.20546/ijcmas.2020.902.180


Isolation, Identification and Antibiotic Susceptibility Testing of Pseudomonas aeruginosa and Acinetobacter baumannii from Endotracheal Secretions in a Tertiary Care Centre
Seba Miriam David1, Chithra Jayaprakash2* and Anna Mathew3
1Interne MOSC Medical College, Kolenchery, Kerala-682311, India
2Department of Microbiology, MOSC Medical College, Kolenchery,
Kerala, 682311, India
3Department of Pharmacology, MOSC Medical College, Kolenchery, Kerala, 682311, India
*Corresponding author
Abstract:

Pseudomonas aeruginosa and Acinetobacter baumanii are aerobic non fermenting gram negative opportunistic pathogens that are major causes of nosocomial infections. Both these microorganisms thrive well in nutrient-deprived conditions and are responsible for a wide spectrum of infections. They show a higher predilection for infection in patients with diminished host defenses. Intensive care units are considered the epicenter of antibiotic resistant infections. Outbreaks are mainly associated with invasive procedures, cross infection via the hands of health staff and prolonged use of broad spectrum antibiotics. The rapidly spreading nature of carbapenem resistance among these organisms has raised concern over the reliability of carbapenems in managing critically ill patients. Successful management of multidrug resistant infections depends on strict compliance to infection control measures and judicious antibiotic use.  The main aim of this study includes to isolate and identify Pseudomonas aeruginosa and Acinetobacter baumannii from endotracheal secretions and perform their antibiotic susceptibility test. Also to establish the prevalence of these organisms in ventilated patients in intensive care units. Approval was obtained from the ethics committee and waiver of informed consent was sought. The samples for this cross-sectional study were endotracheal secretions from patients, processed using standard microbiological procedures and antibiotic susceptibility testing done using Kirby-Bauer disc diffusion method. After incubation the results were read and interpreted as per CLSI guidelines. Non-fermenters constituted 60.24% of the isolates recovered from the 83 endotracheal secretion samples. Pseudomonas aeruginosa was the most common non- fermenter isolated (54%), followed by Acinetobacter baumanii (30%). Pseudomonas aeruginosa was the most commonly isolated non-fermenter (54%) followed by Acinetobacter baumannii(30%). From our study, aminoglycosides like amikacin and gentamicin were found to have good efficacy against Pseudomonas aeruginosa. Acinetobacter baumannii remained highly resistant to most of the antibiotics tested except colistin and tigecycline.


Keywords: Pseudomonas aeruginosa, Acinetobacterbaumanii, Non-ferementing bacilli, Antibiotic susceptibility, Tigecycline, Colistin
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How to cite this article:

Seba Miriam David, Chithra Jayaprakash and Anna Mathew. 2020. Isolation, Identification and Antibiotic Susceptibility Testing of Pseudomonas aeruginosa and Acinetobacter baumannii from Endotracheal Secretions in a Tertiary Care Centre. Int.J.Curr.Microbiol.App.Sci. 9(2): 1566-1574. doi: https://doi.org/10.20546/ijcmas.2020.902.180