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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(2): 1557-1565
DOI: https://doi.org/10.20546/ijcmas.2020.902.179


Formulation of Alternative and Cost Effective Growth Medium for Beneficial Microorganisms using Post Biomethanated Distillery Spentwash
Malarvizhi* and P. Subramanian
Department of Environmental Sciences, TNAU, Coimbatore-641 003, India
*Corresponding author
Abstract:

The increasing cost of microbial growth media has necessitated continuous search for more readily available culture media at affordable prices. The agricultural based industries are generate significant quantities of organic wastes and it is necessary to convert them to useful end products. It is now realized that these waste could be utilized as cheap raw materials for some industries or used as cheap substrates for microbiological processes. Distillery is one of the promising industries in India and in recent years its growth is phenomenal which besides alcohol production, generates enormous quantity of wastewater. Distillery wastes are rich sources of organic matter and nutrients especially potassium, calcium, magnesium and sodium. There is an increasing interest in distillery spentwash due the possibility to use it as a cheap carbon and nutrient source and the presence of several other compounds. These include minerals, organic compounds and vitamins, and this makes the modified spentwash medium as an inexpensive and economic alternative media for Bio inoculants growth. Now an attempt has been made to modify the distillery spentwash to support the beneficial microorganism growth. Comparative studies of Azospirillum sp growth in modified spentwash medium and N-free Malic acid broth were recorded with 24hrs time between 0 to 168 hrs. Maximum population of Azospirillum in N- free Malic acid and modified Spentwash medium was 2.0 x 107 of CFU ml-1 and 2.5 x 107 CFU ml -1 at 120 hrs interval respectively. The Azospirillum sp. colonies present in modified spentwash were showed positive growth confirmation with various sources of carbon and methyl red biochemical test but negative in the voges test. Azospirillum grown in modified spentwash medium produced the maximum amount of IAA (5.6 mg L-1).


Keywords: Post Biomethanated Distillery Spentwash, Modified spentwash medium, Azospirillum
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How to cite this article:

Malarvizhi, C. and Subramanian, P. 2020. Formulation of Alternative and Cost Effective Growth Medium for Beneficial Microorganisms using Post Biomethanated Distillery Spentwash.Int.J.Curr.Microbiol.App.Sci. 9(2): 1557-1565. doi: https://doi.org/10.20546/ijcmas.2020.902.179