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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(2): 1326-1342
DOI: https://doi.org/10.20546/ijcmas.2020.902.156


Partial Purification and Characterization of Proteases from the Visceral Waste of Indian Major Carp, Labeo rohita (Hamilton, 1822)
Biswajit Mohanty*, R. K. Majumdar*, Upasana Mohanty and Naresh Kumar Mehta
College of Fisheries, Central Agricultural University (I), Lembucherra,
Tripura – 799210, India
*Corresponding author
Abstract:

Acidic and alkaline proteases from visceral waste of Labeo rohita (Hamilton, 1822) were isolated, partially purified by ammonium sulphate precipitation followed by dialysis, their kinetics and characteristics studied. The purification fold increased from 1.24 to 2.49 and 1.19 to 1.55 in acidic and alkaline protease respectively along the purification steps. The molecular weight was found in the range of 15-35 kDa and 25-63 kDa respectively in acidic and alkaline proteases. The pH and temperature optima for acidic and alkaline proteases were 3 and 10, at 40°C and 60°C respectively. The Protease activity was decreased by 40% and 60%, when incubated at 90°C for 30 min. Both the proteases showed a decreased activity of more than 50% after incubation with NaCl concentration of 0.5%. Degree of hydrolysis (DH) of the proteases on muscle protein increased with increase of enzyme concentrations. Both soybean trypsin inhibitor and EDTA exhibited high percentage of inhibition, when proteases were incubated with 50 mM of both the inhibitors. The study showed that proteases from Rohu visceral waste of could find use in applications where maximum activity at moderate temperature and low NaCl concentration is desired.


Keywords: Labeo rohita, Acidic proteases, Alkaline proteases, Enzyme purification fold, Enzyme kinetics
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How to cite this article:

Biswajit Mohanty, R. K. Majumdar, Upasana Mohanty and Naresh Kumar Mehta. 2020. Partial Purification and Characterization of Proteases from the Visceral Waste of Indian Major Carp, Labeo rohita (Hamilton, 1822). Int.J.Curr.Microbiol.App.Sci. 9(2): 1326-1342. doi: https://doi.org/10.20546/ijcmas.2020.902.156