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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(2): 1099-1108
DOI: https://doi.org/10.20546/ijcmas.2020.902.128


Detection of Mycoplasma spp. from Choanal and Tracheal Samples using Genus Specific Polymerase Chain Reaction
A. A. Shingade1, R. S. Gandge2, S. B. Majee2, R. J. Zende3, R. R. Pharande2 and S. A. Ingle1*
1Animal Biotechnology Education and Research Cell, Department of Veterinary Microbiology, Mumbai Veterinary College, Parel, Mumbai-400012 (India)
2Department of Veterinary Microbiology, Mumbai Veterinary College, Parel, Mumbai-400012 (India)
3Department of Veterinary Public Health, Mumbai Veterinary College, Parel, Mumbai-400012 (India), India
*Corresponding author
Abstract:

Avian Mycoplasmosis is one of the most substantial global causes of economic losses to the poultry industry, particularly in chickens and turkeys. Present investigation was designed to detect Mycoplasma species from suspected broiler breeder/layer using genus specific Polymerase Chain Reaction (PCR). Hundred specimens including 50 each of choanal swabs and tracheal swabs were collected from five layer/breeder poultry farms from different areas of Maharashtra State. The swabs were inoculated in Pleuropneumonia Like Organism (PPLO) broth for isolation of Mycoplasma organisms. DNA extracted from specimens, broth cultures and reference strains (MG and MS) was amplified by 16S rRNA genus specific PCR yielding ~714bp products. Out of 100, 44/50 (88%) choanal swabs, 50/50 (100%) tracheal swabs were found positive for Mycoplasma spp., while, from broth cultures, 45/50 (90%) choanal swabs, 45/50 (90%) tracheal swabs were found positive by PCR. The 48 hr. old broth cultures yielded better results than PCR from direct specimens. Current study showed that genus specific 16S rRNA PCR was found to be sensitive for detection of Mycoplasma spp. at genus level in direct specimens and broth cultures. Tracheal swabs were better specimens for investigation of Mycoplasma infection in poultry.


Keywords: Avian mycoplasma, genus specific PCR, choanal specimens, tracheal specimens, 16S rRNA,
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How to cite this article:

Shingade. A.A., R. S. Gandge, S. B. Majee, R. J. Zende, R. R. Pharande and Ingle. S.A. 2020. Detection of Mycoplasma spp. from Choanal and Tracheal Samples using Genus Specific Polymerase Chain Reaction.Int.J.Curr.Microbiol.App.Sci. 9(2): 1099-1108. doi: https://doi.org/10.20546/ijcmas.2020.902.128