National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Avian Mycoplasmosis is one of the most substantial global causes of economic losses to the poultry industry, particularly in chickens and turkeys. Present investigation was designed to detect Mycoplasma species from suspected broiler breeder/layer using genus specific Polymerase Chain Reaction (PCR). Hundred specimens including 50 each of choanal swabs and tracheal swabs were collected from five layer/breeder poultry farms from different areas of Maharashtra State. The swabs were inoculated in Pleuropneumonia Like Organism (PPLO) broth for isolation of Mycoplasma organisms. DNA extracted from specimens, broth cultures and reference strains (MG and MS) was amplified by 16S rRNA genus specific PCR yielding ~714bp products. Out of 100, 44/50 (88%) choanal swabs, 50/50 (100%) tracheal swabs were found positive for Mycoplasma spp., while, from broth cultures, 45/50 (90%) choanal swabs, 45/50 (90%) tracheal swabs were found positive by PCR. The 48 hr. old broth cultures yielded better results than PCR from direct specimens. Current study showed that genus specific 16S rRNA PCR was found to be sensitive for detection of Mycoplasma spp. at genus level in direct specimens and broth cultures. Tracheal swabs were better specimens for investigation of Mycoplasma infection in poultry.
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