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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(2): 1069-1075
DOI: https://doi.org/10.20546/ijcmas.2020.902.125


Comparative in-vitro Antibacterial Efficacy of the Different Common Herbs and Antibiotics against E.coli and S.aureus Isolated from Bovine Mastitis
Ravi Sikrodia1*, Daljeet Chhabra1, Rakhi Gangil1, S.D. Audarya1, Rakesh Sharda1 and S.S. Mahor2
1Veterinary Microbiology, COVs & AH, Mhow, India
2Veterinary gynecology and obstetrics, COVs & AH, Mhow, India
*Corresponding author
Abstract:

Mastitis is the inflammation of the mammary gland and udder tissue, and is a major endemic disease of dairy cattle. It is also the most costly disease to the dairy industry. Bovine mastitis caused by Escherichia coli (E.coli) and Staphylococcus aureus can ranges from being a subclinical infection of the mammary gland to a severe systemic disease. Antimicrobial resistance is generally not a limiting factor for treatment, but it should be monitored to detect changes in resistance profiles. This increases the importance of substitute among antimicrobial agents that kill or inhibit the growth of microorganisms. Some herbs like neem, turmeric, garlic and tulsi have some bioactive molecules that show antibacterial activity. These agents protect the host from cellular oxidation reactions. In the present sudy, in vitro antimicrobial sensitivity of ethanolic extracts of neem, turmeric, garlic and tulsi leaves have been compared with the different antibiotics (ciprofloxacin, ofloxacin and amoxicillin) against E.coli and S. aureus isolated from the milk samples of bovine suffering with mastitis. The zone of inhibition of different herbal discs and antibiotics against E.coli and S.aureus were seen after incubation of overnight and measured in millimeter. The maximum zone of inhibition among extracts of herbs and antibiotic were found in neem for E.coli followed by turmeric, ofloxacin and ciprofloxacin. The extracts of tulsi, garlic and antibiotic amoxicillin were found resistant against E.coli. While in case of S.aureus the maximum zone was found in ciprofloxacin followed by tulsi, ofloxacin, garlic, neem and amoxicillin. The extract of turmeric was not showing any sensitivity against S.aureus.


Keywords: Herbs, Antibacterial effect, Mastitis, E.coli, S.aureus
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How to cite this article:

Ravi Sikrodia, Daljeet Chhabra, Rakhi Gangil, S.D. Audarya, Rakesh Sharda and Mahor. S.S. 2020. Comparative in-vitro Antibacterial Efficacy of the Different Common Herbs and Antibiotics against E.coli and S.aureus Isolated from Bovine Mastitis.Int.J.Curr.Microbiol.App.Sci. 9(2): 1069-1075. doi: https://doi.org/10.20546/ijcmas.2020.902.125