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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(2): 1045-1050
DOI: https://doi.org/10.20546/ijcmas.2020.902.122


Salmonellosis in Captive Asian Elephants (Elephas maximus) of TamilNadu State, India
A. Senthilkumar1*, M. G. Jayathangaraj2, A. Valli3, A. Thangavelu4, S. Gomathinayagam5 and N. Sribalaji6
1Farmers Training Centre, Theni-625 53, India
2Department of Veterinary Clinical Medicine and Therapeutics,Madras Veterinary College, Chennai-600007, India
3Institute of Animal Nutrition, PGRIAS, Kattupakkam, Chennai-600007, India
4Department of Veterinary Microbiology, 5Department of Veterinary Parasitology, Madras Veterinary College,
Chennai-600007, India
6MSRS, Pottaneri, India
*Corresponding author
Abstract:

The main objective of the present study was isolation and molecular identification of Genus Salmonella in diarrheic captive Asian elephants (Elephas maximus) of Tamil Nadu state. Isolation and identification of Genus Salmonella was carried out in both fresh water and dung samples from diarrhoeic cases of captive elephants (n=8), using culturing method and PCR technique. Out of the dung (n=8) and water (n=8) samples, 25.00% (n=2) of water samples as well as 25.00% (n=2) of the dung samples were found to be positive for Genus Salmonella organisms. A red coloured colony-growth on modified Brilliant Green Agar and Colourless colony with Black spot at centre indicated the growth of Salmonella. Molecular identification of Genus Salmonella by Polymerase Chain Reaction (PCR), the amplicon size was 260 bp. These findings in this study indicated the need for supply of good quality water to the captive elephants, in order to minimize the possible incidences of contamination of Genus Salmonella.


Keywords: Asian elephants-diarrhoea-Salmonella
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How to cite this article:

Senthilkumar. A., M. G. Jayathangaraj, A. Valli, A. Thangavelu, S. Gomathinayagam and Sribalaji. N. 2020. Salmonellosis in Captive Asian Elephants (Elephas Maximus) of TamilNadu State.Int.J.Curr.Microbiol.App.Sci. 9(2): 1045-1050. doi: https://doi.org/10.20546/ijcmas.2020.902.122