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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(3): 13-23
DOI: https://doi.org/10.20546/ijcmas.2017.603.002


Detection of Grasserie Virus, BmNPV in the Fifth Instar Larvae of Silkworm, Bombyx mori (L) (Race: PM x CSR2) Through Polymerase Chain Reaction
Vitthalrao B. Khyade1* and Brij Kishor Tyagi2
1Sericulture Unit, Malegaon Sheti Farm, Agricultural Development Trust Baramati, Shardanagar, (Malegaon Khurd) Post Box No.:35, Baramati, Pune 413 115, Maharashtra, India
2Department of Zoology and Environment Science, Punjabi University Patiala (Punjab), India
*Corresponding author
Abstract:

The objective of this study to find out the effect of ketamine in combination of opioids on haemodianamics and electrocardiograph in dogs. The ketamine hydrochloride @3mg/kg bwt was administered epidurally at lumbosacrcal space in group I, whereas buprenorphine @0.005 mg/kgbwt, pentazocine @0.3 mg/kg bwt and meperidine hydrochloride (@1 mg/kgbwt were given epidurally in combination with ketamine hydrochloride in group II, III and IV, respectively. To accomplish epidural block, an 18-gauge 3.5 cm hypodermic needle was inserted percutaneously at the prepared site into the epidural space to inject analgesic agent. Haemodynamic and electrocardiographic changes were recorded before and at the time intervals of 5, 15, 30, 60 and minutes of ketamine administration. Haemodyanamic parameters viz.heart rate, systolic arterial pressure, diastolic arterial pressure and mean arterial pressure showed a significant higher (P<0.05) value at initial intervals of observation, thereafter, the values revealed a decreasing trends and returned to base line value by the end of observation. Shortening of PR, R.R. and QT intervals were recorded at 5 min up to 30 min. in all the groups. The QRS complex did not show significant variation among and between group. In conclusion, Ketamine in combination of buprenorphine, pentazocine and meperidine as epidural anaesthesia in dogs was found to be effective and produces reversible changes on haemodynamics and Electrocardiograph.


Keywords: Bombyx mori L,Grasserie Virus, BmNPV,Polymerase Chain Reaction
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How to cite this article:

Vitthalrao B. Khyade and Brij Kishor Tyagi. 2017. Detection of Grasserie Virus, BmNPV in the fifth instar larvae of silkworm, Bombyx mori (L) (Race: PM x CSR2) Through Polymerase Chain Reaction.Int.J.Curr.Microbiol.App.Sci. 6(3): 13-23. doi: https://doi.org/10.20546/ijcmas.2017.603.002