National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Regeneration protocol is pre-requisite for the development of transgenic plants. Maize (Zea mays L) crop is severely affected by fungal disease turcicum leaf blight. In the present study, standardizing the regeneration protocol for maize genotype was carried for the development of transgenic maize against turcicum leaf blight. The direct regeneration of maize involved nodal explants from the maize coleoptiles. Three Turcicum leaf blight susceptible maize genotypes (NAI179, NAI193, and HKI413) were selected for this study. Different combinations of growth regulators were used with MS media to induce shoots and roots. The best responding explants for the shooting was recorded in NAI179 on MS + 1.5mgL-1 BAP media. Fastest responding genotype was found to be HKI413 on MS + 0.5 mgL-1 BAP media. The maximum number of shoots were recorded in NAI179 on MS + mgL-1 BAP media and maximum shoot elongation was seen in NAI193 on MS + 2.5 mgL-1 BAP + 0.5 mgL-1 IBA. The best response for rooting was observed in NAI179 on MS + 0.5 mgL-1 BAP + 2.0 mgL-1 NAA media and fastest responding genotypes was found to be HKI413.
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