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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(2): 241-251
DOI: https://doi.org/10.20546/ijcmas.2020.902.031


Direct Regeneration of Three Indian Maize Genotypes by Multiple Shoot Induction Using Split Nodes
D. P. Adarsha* and N. Nagesha
Department of Plant Biotechnology, College of Agriculture University of Agricultural Sciences, GKVK, Bengaluru – 560 065, India
*Corresponding author
Abstract:

Regeneration protocol is pre-requisite for the development of transgenic plants. Maize (Zea mays L) crop is severely affected by fungal disease turcicum leaf blight. In the present study, standardizing the regeneration protocol for maize genotype was carried for the development of transgenic maize against turcicum leaf blight. The direct regeneration of maize involved nodal explants from the maize coleoptiles. Three Turcicum leaf blight susceptible maize genotypes (NAI179, NAI193, and HKI413) were selected for this study. Different combinations of growth regulators were used with MS media to induce shoots and roots. The best responding explants for the shooting was recorded in NAI179 on MS + 1.5mgL-1 BAP media. Fastest responding genotype was found to be HKI413 on MS + 0.5 mgL-1 BAP media. The maximum number of shoots were recorded in NAI179 on MS + mgL-1 BAP media and maximum shoot elongation was seen in NAI193 on MS + 2.5 mgL-1 BAP + 0.5 mgL-1 IBA. The best response for rooting was observed in NAI179 on MS + 0.5 mgL-1 BAP + 2.0 mgL-1 NAA media and fastest responding genotypes was found to be HKI413.


Keywords: Maize (Zea mays), Regeneration, Split nodes, Multiple shoots, Genotypes
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How to cite this article:

Adarsha, D. P. and Nagesha, N. 2020. Direct Regeneration of Three Indian Maize Genotypes by Multiple Shoot Induction Using Split Nodes.Int.J.Curr.Microbiol.App.Sci. 9(2): 241-251. doi: https://doi.org/10.20546/ijcmas.2020.902.031