National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Mastitis is the most economically significant disease of dairy animals. This condition is associated with a significant reduction in milk yield, increased costs of production and deteriorated milk quality. In the present study a total number of 40 bovine mastitis milk samples were collected to isolate and identify the Pseudomonas aeruginosa. All the samples were inoculated into nutrient broth which was further cultured on agar plates. Colonies showing typical Pseudomonas morphological characteristics were processed for biochemical analysis. Based on the morphological, cultural and biochemical characterization indicated that 6 isolates were identified as Pseudomonas aeruginosa. Investigations of the Pseudomonas aeruginosa proteolysis activity is the basal objective of this study. To achieve this objective 16S rRNA primer was used in the amplification of DNA extracted from bacterial isolates. In the present study six bacterial isolates were subjected to molecular identification. The 16SrRNA based PCR results, revealed 956bp amplicon sized product.
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