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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(2): 194-199
DOI: https://doi.org/10.20546/ijcmas.2020.902.024


PCR Based Detection of Pseudomonas aeruginosa in Mastitic Cow Milk
T. Lurthu Reetha*, R. Manickam and B. Puvarajan
Department of Veterinary Microbiology, Veterinary College and Research Institute, Orathanadu, Thanjavur, India
*Corresponding author
Abstract:

Mastitis is the most economically significant disease of dairy animals. This condition is associated with a significant reduction in milk yield, increased costs of production and deteriorated milk quality. In the present study a total number of 40 bovine mastitis milk samples were collected to isolate and identify the Pseudomonas aeruginosa. All the samples were inoculated into nutrient broth which was further cultured on agar plates. Colonies showing typical Pseudomonas morphological characteristics were processed for biochemical analysis. Based on the morphological, cultural and biochemical characterization indicated that 6 isolates were identified as Pseudomonas aeruginosa. Investigations of the Pseudomonas aeruginosa proteolysis activity is the basal objective of this study. To achieve this objective 16S rRNA primer was used in the amplification of DNA extracted from bacterial isolates. In the present study six bacterial isolates were subjected to molecular identification. The 16SrRNA based PCR results, revealed 956bp amplicon sized product.


Keywords: Cattle, Bovine Mastitis, Pseudomonas aeruginosa, PCR
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How to cite this article:

Lurthu Reetha, T., R. Manickam and Puvarajan, B. 2020. PCR Based Detection of Pseudomonas aeruginosa in Mastitic Cow Milk.Int.J.Curr.Microbiol.App.Sci. 9(2): 194-199. doi: https://doi.org/10.20546/ijcmas.2020.902.024