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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(2): 1902-1907
DOI: http://dx.doi.org/10.20546/ijcmas.2017.602.215


GyrA Mutations in Nosocomial Ciprofloxacin-Resistant Escherichia coli Isolates Associated with Urinary Tract Infections
Rasha H. El-Mahdy1, Mohammed A. Saleh2* and Aalaa Aboelnour3
1Department of Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Egypt 2Department of Medical Microbiology and Immunology, Faculty of Medicine, Al-Azhar University, New Damietta, Egypt 3Department of Clinical Pathology, Faculty of Medicine, Mansoura University, Egypt
*Corresponding author
Abstract:

Escherichia coli (E. coli) is one of most common organisms responsible for health care-associated urinary tract infection (HAUTI). The aim of this study was to determine antibiotic susceptibility pattern of E. coli isolated from HAUT I and to study ciprofloxacin resistance caused by gyrA gene mutations among these isolates. Urine Samples were collected from patients with suspected HAUTI. E. coli identification and antimicrobial susceptibility testing, minimum inhibitory concentration (MIC) of ciprofloxacin were done followed by detection of mutations in the gyrA gene by PCR-RFLP. Eighty seven isolates E. coli were isolated from HAUTI. Thirty-six isolates (41.3%) isolates were ciprofloxacin resistant. Double mutations at gyrA were detected at positions 83 and 87 of the quinolone resistance determining regions (QRDRs) in 25 (69.4%) ciprofloxacin resistant isolates, while single mutation at positions 87 revealed in 9 (25%) isolates. The pressure of the abuse of ciprofloxacin shares significantly to their resistance among E. coli isolated from urinary tract with double mutations at gyrA at positions 83 and 87 of the QRDRs represents an important factor for resistance.


Keywords: Ciprofloxacin Resistant, E.coli, and gyrA Mutations
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How to cite this article:

Rasha H. El-Mahdy, Mohammed A. Saleh and Aalaa Aboelnour. 2017. GyrA Mutations in Nosocomial Ciprofloxacin-Resistant Escherichia coli Isolates Associated with Urinary Tract Infections.Int.J.Curr.Microbiol.App.Sci. 6(2): 1902-1907. doi: http://dx.doi.org/10.20546/ijcmas.2017.602.215