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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(1): 2645-2655
DOI: https://doi.org/10.20546/ijcmas.2020.901.300


Mutagenic Effects of Gamma Rays and EMS in M1 and M2 Generations in Rice (Oryza sativa L.)
Amita Sharma*, S. K. Singh, Rajesh Singh, P. K. Bhati and M. K. Meena
Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi – 221005, India
*Corresponding author
Abstract:

Mutation breeding programme(s) aims to enlarge the frequency and spectrum of mutations and to increase the frequency of viable mutations. The cultivar BPT-5204 revealed maximum reduction in germination (69.00%), seedling height reduction (52.54%), plant survival at maturity (62.50%), pollen sterility (32.82%) and spikelet sterility (29.80%) and Adamchini exhibited maximum reduction in germination (62.33%), seedling height reduction (65.71%), plant survival at maturity (50.90%), pollen sterility (50.86%) and spikelet sterility (34.80%) at a combined dose of 30 kR + 0.02 M EMS. The maximum and minimum frequency of mutation per 100 M2 seedlings was recorded for a combined dose of 30 kR + 0.02 M EMS and 10 kR doses of gamma rays in BPT-5204 and Adamchini respectively. The spectrum of chlorophyll mutations consisted of only four mutant types, viz., albina, xantha, viridis and striata. In the present investigation, albina mutant occurred at higher frequencies and Adamchini recorded the highest frequency of chlorophyll mutations indicating varietal responses.


Keywords: Chlorophyll, EMS, gamma rays, mutation, rice
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How to cite this article:

Amita Sharma, S. K. Singh, Rajesh Singh, P. K. Bhati and Meena M. K. 2020. Mutagenic Effects of Gamma Rays and EMS in M1 and M2 Generations in Rice (Oryza sativa L.).Int.J.Curr.Microbiol.App.Sci. 9(1): 2645-2655. doi: https://doi.org/10.20546/ijcmas.2020.901.300