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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(1): 2518-2523
DOI: https://doi.org/10.20546/ijcmas.2020.901.286


Molecular and Phylogenetic Analysis of Mycoplasma gallisepticum in Haryana, India
Vaishali1*, Davinder Singh2 and Renu Gupta1
1Department of Veterinary Public Health and Epidemiology, LUVAS, Hisar, India
2Extension Specialist, Directorate of Extension Education, LUVAS, Hisar, India
*Corresponding author
Abstract:

Avian mycoplasmosis is responsible for heavy economic loses to the poultry industry in  India. It results from a complex interaction of various factors like viral, bacterial and housing conditions. Mycoplasma gallisepticum (MG) belongs to class Mollicutes is small in size, lacks bacterial cell wall with minimum genetic information. It is the principle pathogenic agent causing Chronic Respiratory disease (CRD) in chickens and infectious sinusitis in Turkeys and has a worldwide distribution.  The objective of this study was molecular detection of Mycoplasma gallisepticum in poultry with respiratory affections in Haryana (India). 100 tissue samples including trachea, lungs and air sacs were collected and pooled from 100 different poultry flocks in different districts of Haryana. The samples were screened by Polymerase chain reaction (PCR) for Mycoplasma gallisepticum. A prevalence of 16% (16/100) was seen for Mycoplasma gallisepticum by using 16S to 23 S rRNA primer. PCR can be used for a simple and quick way to identify Mycoplasma gallisepticum from field samples.


Keywords: Mycoplasma gallisepticum, Molecular and Phylogenetic Analysis
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How to cite this article:

Vaishali, Davinder Singh and Renu Gupta. 2020. Molecular and Phylogenetic Analysis of Mycoplasma gallisepticum in Haryana, India.Int.J.Curr.Microbiol.App.Sci. 9(1): 2518-2523. doi: https://doi.org/10.20546/ijcmas.2020.901.286