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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(1): 2352-2358
DOI: https://doi.org/10.20546/ijcmas.2020.901.268


The Relationship of CD4 Cells and History of Diarrhea to
Cryptosporidium sp. and Giardia lamblia Infections in HIV/AIDS Patients
in West Sumatra Indonesia and its DNA Isolation
Nuzulia Irawati1, Goldha Faroliu2* and Zahra Frizki Asty2
1Parasitology Laboratory, Faculty of Medicine, Andalas University, Padang 25138, Indonesia
2Biomedical Sains, Faculty of Medicine, Andalas University, Padang 25138, Indonesia
*Corresponding author
Abstract:

Opportunistic infections are infections that occur due to a decrease in the immune system and can cause serious conditions in people with weak immune systems such as in people with HIV / AIDS. Cryptosporidium sp. and Giardia lamblia are examples of the opportunistic intestinal protozoa that can cause chronic diarrhea so that it becomes dehydrated and malnutrition which can increase mortality and morbidity in HIV sufferers. Observational study with cross-sectional design on 50 fecal samples of HIV / AIDS patients. Cryptosporidium sp. and Giardia lamblia infections tested microscopically using Ziehl Neelsen's staining and molecular examination with DNA isolation,and looking at the relationship between CD4 cell counts and history of diarrhea with Cryptosporidium sp.and Giardia lamblia infections. The results obtained 2 respondents (4%) positive infected with Cryptosporidium sp., There is a significant relationship between CD4 cell counts with Cryptosporidium sp. infection, p-value=0.001 (p<0.05) and there is a significant relationship between Cryptosporidium sp.infections with a history of diarrhea, p-value=0.045 (p<0.05). And obtained 1 respondents (2%) positive infected with Giardia lamblia, There is no significant relationship between CD4 cell counts with Giardia lamblia. infection, p-value=1.000 (p>0.05) and there is a significant relationship between Giardia lamblia infections with a history of diarrhea, p-value=0.031 (p<0.05).18 out of 50 samples have been successfully carried out the process of isolating DNA specifically thick cell walled microorganisms.


Keywords: Cryptosporidium sp., Giardia lamblia, history of diarrhea, HIV/AIDS, CD4 cells
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How to cite this article:

Nuzulia Irawati, Goldha Faroliu and Zahra Frizki Asty. 2020. The Relationship of CD4 Cells and History of Diarrhea to Cryptosporidium sp. and Giardia lamblia Infections in HIV/AIDS Patients in West Sumatra Indonesia and Its DNA Isolation.Int.J.Curr.Microbiol.App.Sci. 9(1): 2352-2358. doi: https://doi.org/10.20546/ijcmas.2020.901.268