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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(1): 2070-2076
DOI: https://doi.org/10.20546/ijcmas.2020.901.235


Simple Method of Purification of Humic Acid isolated from Press mud
Shreelatha1, S. N. Bhat1*, S.R. Balanagoudar1, R.V. Beladhadi1,Satyanarayana Rao2 and M.V. Ravi1
1Department of Soil Science and Agril. Chemistry, University of Agricultural Sciences, Raichur- 584 104(Karnataka), India
2Department of Agronomy, University of Agricultural Sciences,
Raichur- 584 104(Karnataka), India
*Corresponding author
Abstract:

A study on purification of humic acid (HA) isolated from press mud(PM) was conducted at Organic Farming Research Institute( OFRI), UAS, Raichur during 2017-18 with distilled water as a purifying agent in the place of HCl-HF mixture to purify isolated HA from press mud. The conventional procedure makes use of mineral acids and dialysis step. Standard procedures were used to extract and fractionate HA from press mud. The extracted HA was purified by suspending the HA in  50 ml of distilled water, centrifuged for one hour at an interval of 15 minutes; the supernatant solution was analyzed for K+, Na+ and Ca + Mg by Flame Photometer and Versanate titration methods, respectively. The suspension left over after centrifugation was purified HA which was oven dried at 40 ºC to a constant weight. Washing of HA for 4 consecutive times (15 min for each washing) reduced the ash (mineral matter) content of the HA to an acceptance level of 2 per cent. This finding was associated with significant decrease in content of K+, Na+ and Ca + Mg with increasing washing time. This observation also suggests that the distilled water used during the purification process served as Bronsted-Lowry acid thereby donating more H+ which may have replaced some of K+, Na+ and Ca + Mg at the exchange sites of the HA. Humic acids isolated from press mud can effectively be purified within one hour using distilled water.


Keywords: Humic acid,press mud, purification
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How to cite this article:

Shreelatha, S. N. Bhat, S.R. Balanagoudar, R.V. Beladhadi, Satyanarayana Rao and M.V. Ravi. 2020. Simple Method of Purification of Humic Acid isolated from Press mud. Int.J.Curr.Microbiol.App.Sci. 9(1): 2070-2076. doi: https://doi.org/10.20546/ijcmas.2020.901.235