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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(2): 1853-1863
DOI: http://dx.doi.org/10.20546/ijcmas.2017.602.209


Analysis of Formulated Cattle-Feed Samples for the Natural Occurrence of Storage Fungi and Aflatoxin B1

Jayaraman*, V. Rajesh and K. Vadamalai Krishnan

PG and Research Department of Botany, Government Arts College for Men (Autonomous) Nandanam, Chennai 600 035, India
*Corresponding author
Abstract:

Contamination of feed materials with aflatoxins is an important issue due to both acute and chronic intoxication in animals and also harmful to humans. In the present study, 25 formulated cattle feed samples of different sources were collected from Tamil Nadu and analysed for moisture content, storage mycoflora including aflatoxigenic A. flavus and aflatoxin B1. The results show that the moisture content of the cattle feed samples varied from 7.0% to 14.6% with an average of 10.34%. The storage fungi occurred in the range of 0 to 1,600 cfu/g with an average of 340 cfu/g. Different species of storage fungi occurred in the cattle feed samples are A. glaucus, A. terreus, A. niger, Penicillium speices, A. flavus, A. fumiatus and A. candidus in the order of dominance. Among the species individual fungi, A. glaucus occurred frequently which is a remarkable incidence in the samples collected. Out of 25 samples analysed for the presence of aflatoxin B1, 20 were positive for aflatoxin B1 and 5 were negative.  The quantity of aflatoxin B1 in cattle feed samples ranging from 5 ppb to 60 pbb with an average of 20 pbb.  Out of 10 A. flavus strains isolated 5 were found produced aflatoxin B1.  It is concluded that from the study as the majority of cattle feed samples analysed from the market is observed to be safe level for the contamination with aflatoxinB1 and storage fungi.


Keywords: Cattle feed, aflatoxin B1, Storage fungi, Aspergillus, Penicillium.
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How to cite this article:

Jayaraman, P., V. Rajesh and Vadamalai Krishnan, K. 2017. Analysis of Formulated Cattle-Feed Samples for the Natural Occurrence of Storage Fungi and Aflatoxin B1.Int.J.Curr.Microbiol.App.Sci. 6(2): 1853-1863. doi: http://dx.doi.org/10.20546/ijcmas.2017.602.209