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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(1): 1920-1927
DOI: https://doi.org/10.20546/ijcmas.2020.901.215


Occurrence of Extended Spectrum Beta Lactamase Producing Escherichia coli in Hyena from Chhattisgarh, India
Chandrahas Sannat1*, Anil Patyal2, Nidhi Rawat1, Jasmeet Singh3, Choodamani Chandrakar2, Sanjay Shakya2 and S. D. Hirpurkar1
1Department of Veterinary Microbiology
2Department of Veterinary Public Health and Epidemiology, College of Veterinary Science & A.H., Anjora
3Wildlife Health and Forensic center, Chhattisgarh Kamdhenu Vishwavidyalaya, Durg, Chhattisgarh, INDIA
*Corresponding author
Abstract:

Present study was aimed to characterize Escherichia coli isolated from wound from a hyena. Pus swab collected from wound was processed for bacterial isolation and identification as per standard protocol. Isolate was then subjected to antimicrobial susceptibility test by disc diffusion method using commercial available antibiotics such as ampicillin, amoxycillin, amoxyclav, trimethoprim, chloramphenicol, ciprofloxacin, enrofloxacin, cefepime, cefotaxime, ceftriaxone, ceftriaxone/tazobactum, gentamicin and oxytetracycline. Further isolate was screened for presence of extended spectrum beta lactamase genes (blaSHV, blaTEM and blaCTX-M) by PCR. Bacterial culture, staining and biochemical properties revealed the presence of Escherichia coli. Escherichia coli was found susceptible to chloramphenicol and intermediate sensitive to ceftriaxone/ tazobactum and gentamicin and resistant to ampicillin, amoxycillin, amoxyclav, trimethoprim, ciprofloxacin, enrofloxacin, cefotaxime, cefepime, ceftriaxone and oxytetracycline. Escherichia coli isolate was found positive for blaCTX-M genes whereas blaSHV and blaTEM genes were not detected. In conclusion, investigation of present study revealed the presence of multi drug resistant and extended spectrum beta lactamase producing Escherichia coli in hyena.


Keywords: Hyena, Multi drug resistant Escherichia coli, ESBL, Chhattisgarh
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How to cite this article:

Chandrahas Sannat, Anil Patyal, Nidhi Rawat, Jasmeet Singh, Choodamani Chandrakar, Sanjay Shakya and Hirpurkar, S. D. 2020. Occurrence of Extended Spectrum Beta Lactamase Producing Escherichia coli in Hyena from Chhattisgarh, India.Int.J.Curr.Microbiol.App.Sci. 9(1): 1920-1927. doi: https://doi.org/10.20546/ijcmas.2020.901.215