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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(1): 1706-1710
DOI: https://doi.org/10.20546/ijcmas.2020.901.188


Detection of MBL among Carbapenem resistant Enterobacteriaceae
in a tertiary hospital using combined disc test
Martin M. Varghese*, Rama Sikka, Kausalya Raghuraman and Aparna Yadav
Department of Microbiology Pt. B. D. Sharma Post graduate Institute of Medical Sciences, Rohtak, Haryana, India
*Corresponding author
Abstract:

MBL producing members of family Enterobacteriaceae have been responsible for numerous outbreaks of infection throughout the world. A total of 108 non repetitive carbapenem resistant strains isolated from various clinical samples, identified by standard microbiological techniques and antimicrobial susceptibility pattern was determined. MBL detection was done by combined disc test using ceftazidime-EDTA, imipenem-EDTA and meropenem-EDTA. Ceftazidime-EDTA detected 90 (97.8%) isolates, imipenem-EDTA detected 14 (15.2%) isolates and meropenem- EDTA detected 13 (14.1%) isolates showing MBL production. The combined disc test using inhibitors like EDTA was found to be sensitive in detecting MBL producers, although the results vary with different antibiotic discs used. Detection of MBL among Carbapenem resistant Enterobacteriaceae in a tertiary hospital using combined disc test.


Keywords: carbapenem, resistant, Enterobacteriaceae, MBL, CDT
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How to cite this article:

Martin M. Varghese*, Rama Sikka, Kausalya Raghuraman and Aparna Yadav. 2020. Detection of MBL among Carbapenem resistant Enterobacteriaceae in a tertiary hospital using combined disc test. Int.J.Curr.Microbiol.App.Sci. 9(1): 1706-1710. doi: https://doi.org/10.20546/ijcmas.2020.901.188