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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(1): 1473-1484
DOI: https://doi.org/10.20546/ijcmas.2020.901.164


The Etiological Study of Fungal Keratitis in a tertiary Hospital of Mandya District, Karnataka, India
Khalid Ahmed1* and B. Sumangala2
1Department of Microbiology, KMCT medical college, Manassery, Calicut 673601. India
2Department of Microbiology, MIMS, Mandya-571401, India
*Corresponding author
Abstract:

Corneal blindness is responsible for 1.5 to 2 million new cases of monocular blindness every year in which ocular trauma and corneal ulceration are significant contributors. Corneal infections of fungal etiology are common in India (19-45%). Aspergillus (16–53%) and Fusarium (10–45%) species are the common pathogens. Purpose: 1.To Identify and isolate the various fungi causing fungal keratitis. 2. To know the usefulness of Gram stain, KOH wet mount and fungal culture in identification of fungi. Materials and Methods: After taking written and informed consent taken. Examination by an Ophthalmologist was done. Multiple corneal scrapings were collected using Bard-Parker blade no 15. Scrapings were collected and used in a sequence first to inoculate on a set of SDA and Blood agar plates, then scrapings were placed on 2 different clean and labelled glass slides for KOH wet mount, Grams stain. Molds were Identified on the basis of colony morphology such as growth rate, colony texture, color, diffusible pigment , and aerial or vegetative hyphae. Results: Out of 68 study subjects, 18 (26.4%) were culture positive. 45 males and 23 were females. Mean Age was 40. Fusarium sp was predominant (44.4%) followed by Aspergills sp (38.9%). Conclusion: Fungal keratitis being a sight threatening disorder, early clinical suspicion, rational use of laboratory diagnostic procedures for timely mycological diagnosis and therapy at right time are important.


Keywords: Mycotic keratitis, fungal corneal ulcer, fusarium, C shaped markings
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How to cite this article:

Khalid Ahmed and Sumangala, B. 2020. The Etiological Study of Fungal Keratitis in a tertiary Hospital of Mandya District, Karnataka, India.Int.J.Curr.Microbiol.App.Sci. 9(1): 1473-1484. doi: https://doi.org/10.20546/ijcmas.2020.901.164