National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The desi cotton hybrids viz., G.Cot.DH-7 (Sujay x G 27) and G.Cot.DH-9 (4011 x 824) and their parents were characterized using morphological and molecular markers. The desi cotton hybrids and their parents showed variations for morphological characters viz., foliage, leaf shape, leaf hairiness, growth habit, pollen colour, bolls shape and stem hairiness. Based on the distinct morphological characters one can identify true hybrid. A set of 25 RAPD and 19 ISSR were used for molecular characterization of desi hybrids. Primer ISSR9 amplified two polymorphic loci designated as ISSR9_200bp (Sujay) and ISSR9_550bp (G.27) in G.Cot.DH-7; this heteroallelic marker not only differentiated the parents but also confirmed true hybrid. RPI7 amplified a polymorphic marker of approximately 450 bp and 500 bp in the female parent (4011) and hybrid G.Cot.DH-9 but not in the male parent (824). Similarly, RPI8 primer amplified allele of 550bp in the male parent and the hybrid G.Cot.DH-9, but not in its female parent. Thus, the combination of RPI7_450 (4011) and RPI8_550 (824) marker can be used for identification of G.Cot.DH-9. Our study revealed the parentage and desi hybrid confirmation using molecular marker and the markers identified would be valuable genomic tool for desi cotton breeding programs.
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