Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(1): 1232-1239
DOI: https://doi.org/10.20546/ijcmas.2020.901.136


Genetic Characterization of Desi Cotton Hybrids Using Molecular Markers
Khushbu Patel1, B. K. Rajkumar2*, Preeti R. Parmar2, D. H. Patel2 and B. G. Solanki2
1 Shree Ramkrishna College of Computer Education and Applied Sciences, Surat
2Main Cotton Research Station, Navsari Agricultural University, Athwa Farm, Surat, India
*Corresponding author
Abstract:

The desi cotton hybrids viz., G.Cot.DH-7 (Sujay x G 27) and G.Cot.DH-9 (4011 x 824) and their parents were characterized using morphological and molecular markers. The desi cotton hybrids and their parents showed variations for morphological characters viz., foliage, leaf shape, leaf hairiness, growth habit, pollen colour, bolls shape and stem hairiness. Based on the distinct morphological characters one can identify true hybrid. A set of 25 RAPD and 19 ISSR were used for molecular characterization of desi hybrids. Primer ISSR9 amplified two polymorphic loci designated as ISSR9_200bp (Sujay) and ISSR9_550bp (G.27) in G.Cot.DH-7; this heteroallelic marker not only differentiated the parents but also confirmed true hybrid. RPI7 amplified a polymorphic marker of approximately 450 bp and 500 bp in the female parent (4011) and hybrid G.Cot.DH-9 but not in the male parent (824). Similarly, RPI8 primer amplified allele of 550bp in the male parent and the hybrid G.Cot.DH-9, but not in its female parent. Thus, the combination of RPI7_450 (4011) and RPI8_550 (824) marker can be used for identification of G.Cot.DH-9. Our study revealed the parentage and desi hybrid confirmation using molecular marker and the markers identified would be valuable genomic tool for desi cotton breeding programs.


Keywords: RAPD, ISSR, SSR, Molecular marker, Desi hybrid, Cotton
Download this article as Download

How to cite this article:

Khushbu Patel, B. K. Rajkumar, Preeti R. Parmar, D. H. Patel and Solanki, B. G. 2020. Genetic Characterization of Desi Cotton Hybrids Using Molecular Markers.Int.J.Curr.Microbiol.App.Sci. 9(1): 1232-1239. doi: https://doi.org/10.20546/ijcmas.2020.901.136