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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(1): 1102-1119
DOI: https://doi.org/10.20546/ijcmas.2020.901.125


Chemical Composition and Antibacterial Activity of Fractions from Bridelia micrantha Stem Bark Methanol Extract
Colette ElyseeAboudi Etono, Raymond Simplice Mouokeu,
Guy SedarSingor Njateng, Alembert Tchinda Tiabou, Rebecca Madeleine Ebelle Etame, Rosalie Anne Ngono Ngane1* and MouelleSone Albert
1Faculty of Sciences, University of Douala, P.O. Box 24157, Douala, Cameroon
2Institute of Fisheries and Aquatic Sciences, University of Douala,
P.O. Box 7236, Douala, Cameroon
3Faculty of Sciences, University of Dschang, P.O. Box 67, Dschang, Cameroon
4Institute of Medical Research and Medicinal Plant Studies (IMPM),
P.O. Box 6163, Yaoundé, Cameroon
5Faculty of Medicine and Pharmaceutical Sciences, University of Douala, P.O. Box 2701, Douala, Cameroon
*Corresponding author
Abstract:

Bridelia micrantha commonly known as coastal golden leaf is a member of the family Phyllanthaceae. In preliminary studies, nine fractions, named F1- F9, were obtained by fractionating the crude methanol extract of the stem bark of Bridelia micrantha using column chromatographic techniques. The F6 fraction was found to be the most active when tested for the antibacterial activity. This study is thus aimed at investigating the effect of fractionation on antibacterial activity of F6 fraction. The F6 fraction was fractionated by adsorption chromatography on silica gel into eight sub-fractions designated F’1- F’8. A product was isolated from the dichloromethane/ methanol (10%) fraction and the structure was determined on the basis of spectroscopic data. The antibacterial activity of the F6 fraction, sub-fractions and the product was evaluated by broth microdilution method against two reference strains and eighteen clinical bacterial strains. The chemical analysis of F6 and three sub-fractions F’3, F’4 and F’5 was done using HPLC-MS. The fraction F6exhibited strong activity on all the tested bacteria with MIC values of 128 µg/ml on nine strains, including Pseudomonas aeruginosa, methicillin-resistant Staphylococcus aureus, and Salmonella sp.The sub-fractions F’4 and F’5 exhibited the best activities on all the tested bacteria with MIC values of 32 to 256 µg/ml. The chemical analysis by HPLC-MS of F6, F’3, F’4 and F’5 revealed the presence of almost 180 identified compounds from various classes of secondary metabolites including alkaloids, flavonoids, steroids and terpenoids. The product obtained although inactive was elucidated as Daucosterol.


Keywords: Bridelia micrantha, Methanol extract, Fractionation, Antibacterial activity, Chromatography
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How to cite this article:

Colette ElyseeAboudi Etono, Raymond Simplice Mouokeu, Guy SedarSingor Njateng, Alembert Tchinda Tiabou, Rebecca Madeleine Ebelle Etame, Rosalie Anne Ngono Ngane and MouelleSone Albert. 2020. Chemical Composition and Antibacterial Activity of Fractions from Bridelia micrantha Stem Bark Methanol Extract.Int.J.Curr.Microbiol.App.Sci. 9(1): 1102-1119. doi: https://doi.org/10.20546/ijcmas.2020.901.125