Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(1): 941-952
DOI: https://doi.org/10.20546/ijcmas.2020.901.106


Isolation and Screening of Carotenoid Producing Bacteria
Deepti D. Dhere1*, S. M. Dharmadhikari2 and R. S. Pandhare2
1Deogiri College of Arts, Science and Commerce, Aurangabad, (M.S.), India
2Government College of Arts and Science, Aurangabad, (M.S), India
Affiliated to-Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, (M.S.) India
*Corresponding author
Abstract:

This article includes isolation and screening of carotenoid producing bacteria. Samples were collected from different eco-climatic regions of Maharashtra. From soil, mud, sediment, and water samples total 620 bacterial strains were isolated out of which 105 isolates were pigment producers, many of which exhibited bright orange, orange, yellow orange, lemon yellow and red pigmentation. Out of 105 isolates efficient carotenoid producers were screened after primary, secondary, tertiary, and quaternary screening based on pigmentation, tolerance to pH and NaCl%(w/v), degree of pigmentation, absorption maxima and free radicle scavenging activity respectively. The selection of potential carotenoid producer was done from finally screened isolatesSL2, SL4*, SL4-1, SL10, SL2y, DKMIII, DABW1, DGM, SSD2, SWT3 and SS1 based on free radicle scavenging activity by DPPH (1,1 Diphenyl-2 Picryl Hydrazil) method. Isolate SL2 showed bright orange pigmentation, tolerance to pH (6-12), NaCl (1-6%), λmax at 473 nm and maximum 53% free radicle scavenging activity, so it was selected for further studies as a potential carotenoid pigment producer.


Keywords: Screening, DPPH, Carotenoid producing bacteria
Download this article as Download

How to cite this article:

Deepti D. Dhere, S. M. Dharmadhikari and Pandhare, R. S. 2020. Isolation and Screening of Carotenoid Producing Bacteria.Int.J.Curr.Microbiol.App.Sci. 9(1): 941-952. doi: https://doi.org/10.20546/ijcmas.2020.901.106