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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(1): 884-890
DOI: https://doi.org/10.20546/ijcmas.2020.901.098


Detection of Virulence Genes in Aeromonas sobria Isolated from Diseased Fish Rohu, Labeo rohita by PCR
Chandra Bhushan Kumar*, Shilpa kumari, Anil Kumar, Shivanand,
Chinmayee Muduli, Vikash Sahu, S. M. Srivastava and Gaurav Rathore
ICAR-National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, Telibagh, Lucknow-226002, U.P., India
*Corresponding author
Abstract:

The Genus Aeromonas belongs to facultative, anaerobic gram negative bacteria. Several mesophilic species of Aeromonas cause septicaemia in a variety of aquatic organisms and gastrointestinal diseases in humans. The virulence of Aeromonas is considered multifactorial and are known to possess aerolysins, hemolysins, proteases, phospholipases, lipases, adhesions and enterotoxins. Therefore, the importance of present work lies in detecting the virulence genes in Aeromonas sobria isolated from diseased fish, rohu. The bacterium was identified using VITEK 2, automated bacterial identification system. Out of four genes screened, three genes namely, actA (cytotoxic enterotoxin), ahh1A (extracellular hemolysin) and enoA (enolase) were detected in A. sobria by PCR. However, astA gene which encodes for heat-stable cytotonic enterotoxin was not detected.


Keywords: Aeromonas sobria, Virulence genes, Cytotoxic enterotoxin, Extracellular hemolysin, Enolase, Cytotonic enterotoxin
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How to cite this article:

Chandra Bhushan Kumar, Shilpa kumari, Anil Kumar, Shivanand, Chinmayee Muduli, Vikash Sahu, S. M. Srivastava and Gaurav Rathore. 2020. Detection of Virulence Genes in Aeromonas sobria Isolated from Diseased Fish Rohu, Labeo rohita by PCR.Int.J.Curr.Microbiol.App.Sci. 9(1): 884-890. doi: https://doi.org/10.20546/ijcmas.2020.901.098